1887

Abstract

SUMMARY

Enhancement of endogenous xenotropic virus expression has been found upon treatment with tetrapeptides of a high-passage clone of Balb/c (K-Balb) mouse cells transformed with Kirsten sarcoma virus. Tuftsin (Thr-Lys-Pro-Arg) and kentsin (Thr-Pro-Arg-Lys) increased the expression of virus that was infectious for rat, but not mouse, cells in a concentration-dependent fashion. The enhancement of virus expression by the tetrapeptides was proportional to the spontaneous release of virus. The infectivity of the enhanced virus was neutralized by goat anti-RLV gp70 serum. Actinomycin D inhibited the induction of virus, suggesting that enhanced expression required RNA synthesis. The effects observed using K-Balb cells offer an opportunity to study the many biological effects of these peptides in a fibroblast culture system.

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1981-01-01
2022-08-16
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References

  1. Aaronson S. A., Dunn C. Y. 1974a; High frequency C-type virus induction by inhibitors of protein synthesis. Science 183:422–424
    [Google Scholar]
  2. Aaronson S. A., Dunn C. Y. 1974b; Endogenous C-type viruses of Balb/c cells: frequencies of spontaneous and chemical induction. Journal of Virology 13:181–185
    [Google Scholar]
  3. Aaronson S. A., Stephenson J. R. 1973; Independent segregation of loci for activation of biologically distinguishable RNA C-type viruses in mouse cells. Proceedings of the National Academy of Sciences of the United States of America 70:2055–2058
    [Google Scholar]
  4. Aaronson S. A., Todaro G. J. 1968; Basis for the acquisition of malignant potential by mouse cells cultivated in vitro. Science 162:1024–1026
    [Google Scholar]
  5. Aaronson S. A., Hartley J. W., Todaro G. J. 1969; Mouse leukemia virus: ‘spontaneous’ release by mouse embryo cells after long-term in vitro cultivation. Proceedings of the National Academy of Sciences of the United States of America 64:87–94
    [Google Scholar]
  6. Aaronson S. A., Todaro G. J., Scolnick E. 1971; Induction of murine type C viruses from clonal line of virus-free Balb 3T3 cells. Science 174:157–159
    [Google Scholar]
  7. Aaronson S. A., Anderson G. R., Dunn C. Y., Robbins K. C. 1974; Induction of type C RNA virus by cycloheximide: increased expression of virus-specific RNA. Proceedings of the National Academy of Sciences of the United States of America 71:3941–3942
    [Google Scholar]
  8. Aksamit R. R., Long C. W. 1978; Isolation of thioguanine resistant variants of K-BALB cells non-inducible for type C viruses by 5-iododeoxyuridine. Journal of General Virology 38:419–429
    [Google Scholar]
  9. Besmer P., Smotkin D., Hazeltine W., Fan H., Wilson A., Paskind M., Weinberg R., Baltimore D. 1975; Mechanisms of induction of RNA tumor viruses by halogenated pyrimidine. Cold Spring Harbor Symposia on Quantitative Biology 39:1103–1107
    [Google Scholar]
  10. Cabradilla D. C., Robbins K. C., Aaronson S. A. 1976; Induction of mouse type C virus by translational inhibitors: evidence for transcriptional derepression of a special class of endogenous virus. Proceedings of the National Academy of Sciences of the United States of America 73:4541–4545
    [Google Scholar]
  11. Dunn C. Y., Aaronson S. A., Stephenson J. R. 1975; Interactions of chemical inducers and steroid enhancers of endogenous mouse type C RNA viruses. Virology 66:579–588
    [Google Scholar]
  12. Florentin I., Bruley-Rosset M., Kiger N., Imbach J. L., Winternitz F., Mathe G. 1978; In viVO immunostimulation by tuftsin. Cancer Immunology and Immunotherapy 5:211–216
    [Google Scholar]
  13. Fritzsche H. 1972; DNA-oligopeptid wechselwirkung. NMR-untersuchungen zur spezifitat der oligopeptid-bindung. FEBS Letters 23:105–106
    [Google Scholar]
  14. Helene C., Dimicoli J. 1972; Interaction of oligopeptides and aromatic amino acids with nucleic acids. Fluorescence and protein magnetic resonance studies. FEBS Letters 26:6–10
    [Google Scholar]
  15. Kent H. A. 1975; Contraceptive polypeptide from hamster embryos: sequence of amino acids in the compound. Biological Reproduction 12:504–507
    [Google Scholar]
  16. Klement V., Nicolson M. O., Nelson-Rees W., Gilden R. V., Oroszlan S., Rongey R. W., Gardner M. B. 1973; Spontaneous production of a C-type RNA virus in rat tissue culture lines. International Journal of Cancer 12:654–666
    [Google Scholar]
  17. Lieber M. M., Livingston D. M., Todaro G. J. 1973; Superinduction of endogenous type C virus by 5-bromodeoxyuridine from transformed mouse clones. Science 181:443–444
    [Google Scholar]
  18. Long C. W., Suk W. A., Greenawalt C. 1978; Activation of endogenous type C virus by amino acid alcohols. Virology 88:194–196
    [Google Scholar]
  19. Long C. W., Bruszewski J. A., Snead R. M. 1980; Analysis of transcription during type C viral induction using cell permeabilization techniques. Cancer Research 40:22–25
    [Google Scholar]
  20. Lowy D. R., Rowe W. P., Teich N., Hartley J. W. 1971; Murine leukemia virus: high frequency activation in vitro by 5-iododeoxyuridine and 5-bromodeoxyuridine. Science 174:155–156
    [Google Scholar]
  21. Luftig R. B., Yoshinaka Y., Oroszlan S. 1977; Sequence relationship between Rauscher leukemia virus (RLV) p65-70 (gag gene product) and tuftsin as monitored by the p65-70 proteolytic factor. Journal of Cell Biology 75, 397a: Abstract No. VI562
    [Google Scholar]
  22. Martinez J., Winternitz F., Vindel J. 1977; Nouvelles synthesis et proprietes de la tuftsine. European Journal of Medical Chemistry – Chemica Therapeutica 12:511–516
    [Google Scholar]
  23. Najjar V. A. 1974; The physiological role of γ-globulin. Advances in Enzymology 41:129–178
    [Google Scholar]
  24. Najjar V. A., Nishioka K. 1970; ‘Tuftsin’: a natural phagocytosis stimulating peptide. Nature, London 228:672–673
    [Google Scholar]
  25. Nakata Y., Bader J. P. 1968; Transformation by murine sarcoma virus: fixation (deoxyribonucleic acid synthesis) and development. Journal of Virology 2:1255–1261
    [Google Scholar]
  26. Nishioka K. 1979; Anti-tumour effect of the physiological tetrapeptide, tuftsin. British Journal of Cancer 39:342–345
    [Google Scholar]
  27. Nishioka K., Constantopoulos A., Satoh P. S., Najjar V. A. 1972; The characteristics, isolation and synthesis of the phagocytosis stimulating peptide tuftsin. Biochemical and Biophysical Research Communications AT172–179
    [Google Scholar]
  28. Nishioka K., Constantopoulos A., Satoh P. S., Mitchell W. M., Najjar V. A. 1973a; Characteristics and isolation of the phagocytosis stimulation peptide-tuftsin. Biochimica et Biophysica Acta 310:217–229
    [Google Scholar]
  29. Nishioka K., Satoh P., Constantopoulos A., Najjar V. A. 1973b; The chemical synthesis of the phagocytosis stimulating tetrapeptide tuftsin (Thr-Lys-Pro-Arg) and its biological properties. Biochimica et Biophysica Acta 310:230–237
    [Google Scholar]
  30. Oroszlan S., Henderson L. E., Stephenson J. R., Copeland T. D., Long C. W., Ihle J. N., Gilden R. V. 1978; Amino-acid carboxyl-terminal amino acid sequences of proteins coded by gag gene of murine leukemia virus. Proceedings of the National Academy of Sciences of the United States of America IS1404–1408
    [Google Scholar]
  31. Pickart L., Thaler M. M. 1973; Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nature New Biology 243:85–87
    [Google Scholar]
  32. Pickart L., Thayer L., Thaler M. M. 1973; Synthetic tripeptide which increases survival of normal liver cells, and stimulates growth in hepatoma cells. Biochemical and Biophysical Research Communications 54:562–566
    [Google Scholar]
  33. Stabinsky Y., Gottlieb P., Zakuth V., Spirer Z., Fridkin M. 1978; Specific binding sites for the phagocytosis stimulating peptide tuftsin on human polymorphonuclear leukocytes and monocytes. Biochemical and Biophysical Research Communications 83:599–606
    [Google Scholar]
  34. Stephenson J. R., Reynolds R. K., Tronick S. R., Aaronson S. A. 1975; Distribution of three classes of endogenous type C RNA viruses among inbred strains of mice. Virology 67:404–414
    [Google Scholar]
  35. Todaro G. J., Delarco J. E. 1978; Growth factors produced by sarcoma virus-transformed cells. Cancer Research 38:4147–4154
    [Google Scholar]
  36. Toulme J. J., Helene C. 1977; Specific recognition of single-strand nucleic acids. Interaction of tryptophan-containing peptides with native, denatured, and ultraviolet-irradiated DNA. Journal of Biological Chemistry 252:244–249
    [Google Scholar]
  37. Tzehovel E., Segal S., Stabinsky Y., Fridkin M., Spirer Z., Feldman M. 1978; Tuftsin (an Ig-aSSOCiated tetrapeptide) triggers the immunogenetic function of macrophages: implications for activation of programmed cells. Proceedings of the National Academy of Sciences of the United States of America 75:3400–3404
    [Google Scholar]
  38. Versteegen R., Oroszlan S. 1980; Effect of chemical modification and fragmentation on antigenic determinants of internal protein p30 and surface glycoprotein gp70 of type C retroviruses. Journal of Virology 33:983–992
    [Google Scholar]
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