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Abstract
SUMMARY
The virus isolated from the black beetle, Heteronychus arator, is an icosahedral ribovirus with a diam. of 30 nm and contains one major structural polypeptide of mol. wt. 40000. The virus can be grown in Drosophila melanogaster cells and also titred in this line using a plaque assay method. Infected Drosophila cells pulsed with 35S-methionine in the presence of actinomycin D induced three virus polypeptides of mol. wt. 110000, 40000 and 8000. Short pulses, chases with excess non-radioactive methionine and pre-pulse treatments with protease inhibitors failed to demonstrate processing of virus-induced polypeptides.
- Received:
- Accepted:
- Published Online:
© Journal of General Virology 1981