150 lymphoblastoid cell lines derived from normal individuals or from subjects with various clinical conditions were induced to form interferon by treatment with Sendai virus. Irrespective of the status of the donor, most of the lines produced some interferon and 22 produced considerable amounts (more than 3000 international units/ml). Lines derived from infectious mononucleosis patients were good interferon producers while those from leukaemic donors were poor producers. The data suggest that the clinical conditions of the donor and the source of transforming virus may influence the quantity of interferon produced by a given cell line.
ChristofinisG. J.1970; Biological characteristics of cell line GL-V3 derived from the kidney of a vervet monkey (Cercopithecus Aethiops). Journal of Medical Microbiology 3:251–258
ChristofinisG. J.,
FinterN. B.1977; The preparation of interferon from lymphoblastoid cell lines. Proceedings of the Symposium on Preparation, Standardisation and Clinical Use of Interferon The Yugoslav Academy of Sciences and Arts: Zagreb;
FinterN. B.1969; Dye uptake methods for assaying viral cytopathogenicity and their application to interferon assays. Journal of General Virology 5:419–427
HaaseA. T.,
JohnsonG. S.,
KaselH. A.,
MargolisS.,
LevyH. B.1970; Induction of interferon in lymphoblastoid cell lines. Proceedings of the Society for Experimental Biology and Medicine 133:1076–1083
JohnstonM. D.,
FinterN. B.,
YoungP. A.1980; Dye uptake method for assay in interferon activity. In Interferon Methods in Enzymology Edited by
PestkaS.
New York and London; Academic Press: (in press)
KleinG.,
DombosL.,
GothoskarB.1972; Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB virus. International Journal of Cancer 10:44–57
LeeS. H. S.,
OzereR. L.,
Van RooyenC. E.1966; Interferon production by human leukocytes in vitro. Reduced levels in lymphatic leukaemia. Proceedings of the Society for Experimental Biology and Medicine 122:32–39
SteelC. M.1972; Human lymphoblastoid cell lines. III. Co-cultivation technique for establishment of new lines. Journal of the National Cancer Institute 48:623–628