%0 Journal Article %A Suárez, Horacio G. %A Lange, Michele %A Cassingena, Rolando %T Enhancement of Transcription of the SV40 Genome in Mouse Embryo Cells by Pretreatment with 5-Iodo-2′-deoxyuridine %D 1981 %J Journal of General Virology, %V 52 %N 1 %P 135-139 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-52-1-135 %I Microbiology Society, %X SUMMARY Treatment of mouse embryo (ME) cells with 5-iodo-2′-deoxyuridine (IdUrd) before infection with SV40 virus, enhances T-antigen (T-Ag) production as detected by immunofluorescence and complement fixation. Cellular DNA and RNA synthesis are inhibited in both SV40 and mock-infected cells after IdUrd treatment. The analogue pretreatment significantly increases the amount of radiolabelled nuclear and cytoplasmic SV40-specific RNA and the RNA polymerase activity of the viral transcriptional complexes of the Sarkosyl supernatants, suggesting that the enhancement of SV40 T-Ag production in infected pretreated ME cells results from an increased synthesis of early virus RNA. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-52-1-135