1887

Abstract

Summary

Treatment of mouse embryo (ME) cells with 5-iodo-2′-deoxyuridine (IdUrd) before infection with SV40 virus, enhances T-antigen (T-Ag) production as detected by immunofluorescence and complement fixation. Cellular DNA and RNA synthesis are inhibited in both SV40 and mock-infected cells after IdUrd treatment. The analogue pretreatment significantly increases the amount of radiolabelled nuclear and cytoplasmic SV40-specific RNA and the RNA polymerase activity of the viral transcriptional complexes of the Sarkosyl supernatants, suggesting that the enhancement of SV40 T-Ag production in infected pretreated ME cells results from an increased synthesis of early virus RNA.

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/content/journal/jgv/10.1099/0022-1317-52-1-135
1981-01-01
2019-10-17
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-52-1-135
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