Physical Mapping of Temperature-sensitive Mutations of Herpes Simplex Virus Type 2 by Marker Rescue Free

Abstract

SUMMARY

The physical mapping of six mutations of herpes simplex virus type 2 (HSV-2) is presented. The results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 HSV-1/HSV-2 intertypic recombinants. The order of these mutations on the physical map of HSV-2 is unambiguous and correlates almost exactly with the previously published genetic map of Timbury & Calder (1976). One of the mutants studied (HSV-2 12) has apparently two distinct conditionally lethal mutations, one in the long and the other in the short region of the HSV genome.

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1981-01-01
2024-03-28
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References

  1. Becker Y., Dym H., Sarov Y. 1968; Herpes simplex virus DNA. Virology 36:184–192
    [Google Scholar]
  2. Bookout J. B., Schaffer P. A., Purifoy D. J. M., Biswal N. 1978; Marker rescue of temperature-sensitive mutants by defective DNA of herpes simplex virus type 1. Virology 89:528–538
    [Google Scholar]
  3. Chartrand P., Stow N. D., Timbury M. C., Wilkie N. M. 1979; Physical mapping ofpaa′ mutations of herpes simplex virus type 1 and 2 by intratypic marker rescue. Journal of Virology 31:265–276
    [Google Scholar]
  4. Chartrand P., Crump Acker C. S., Schaffer P. A., Wilkie N. M. 1980; Physical and genetic analysis of the herpes simplex virus DNA polymerase locus. Virology (in press)
    [Google Scholar]
  5. Chu C.-T., Parris D. S., Dixon R. A. F., Farber F. E., Schaffer P. A. 1979; Hydroxylamine mutagenesis of HSV DNA and DNA fragments: introduction of mutations into selected regions of the viral genome. Virology 98:168–181
    [Google Scholar]
  6. Clements J. B., Cortini R., Wilkie N. M. 1976; Analysis of herpes virus DNA substructure by means of restriction endonucleases. Journal of General Virology 30:243–256
    [Google Scholar]
  7. Cortini R., Wilkie N. M. 1978; Physical maps for HSV type 2 DNA with five restriction endonucleases. Journal of General Virology 39:259–280
    [Google Scholar]
  8. Crombie I. 1975 Genetic and biochemical studies with herpes simplex virus type 1 Ph. D. thesis University of Glasgow;
    [Google Scholar]
  9. Delius H., Clements J. B. 1976; A partial denaturation map of herpes simplex virus type 1 DNA: evidence for inversions of the unique DNA regions. Journal of General Virology 33:125–133
    [Google Scholar]
  10. Graham F. L., Van Der Eb A. I. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  11. Halliburton I. W., Timbury M. C. 1973; Characterisation of temperature-sensitive mutants of herpes simplex virus type 2. Growth and DNA synthesis. Virology 52:60–80
    [Google Scholar]
  12. Halliburton I. W., Timbury M. C. 1976; Temperature-sensitive mutants of herpes simplex virus type 2: description of three new complementation groups and studies on the inhibition of host cell DNA synthesis. Journal of General Virology 30:207–221
    [Google Scholar]
  13. Hay J., Moss H., Jamieson A. T., Timbury M. C. 1976; Herpes virus proteins: DNA polymerase and pyrimidine deoxynucleoside kinase activities in temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 31:65–73
    [Google Scholar]
  14. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. 1975; Anatomy of herpes simplex virus DNA: evidence for four populations that differ in the relative orientations of their long and short components. Proceedings of the National Academy of Sciences of the United States ofAmerica 72:4243–4247
    [Google Scholar]
  15. Kieff E. D., Bachenheimer S. L., Roizman B. 1971; Size, composition and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. Journal of Virology 8:125–132
    [Google Scholar]
  16. Knipe D. M., Ruyechan W. T., Roizman B., Halliburton I. W. 1978; Molecular genetics of herpes simplex virus. Demonstration of regions of obligatory and non-obligatory identity within diploid regions of the genome. Proceedings of the National Academy of Sciences of the United States of America 75:3896–3900
    [Google Scholar]
  17. Knipe D. M., Ruyechan W. T., Honess R. W., Roizman B. 1979a; Molecular genetics of herpes simplex virus: the terminal a sequence of the L and S components are obligatorily identical and constitute a part of a structural genes mapping predominantly in the S component. Proceedings of the National Academy of Sciences of the United States of America 76:4534–4538
    [Google Scholar]
  18. Knipe D. M., Ruyechan W. T., Roizman B. 1979b; Molecular genetics of herpes simplex virus. III. Fine mapping of a genetic locus determining resistance to phosphonoacetate by two methods of marker transfer. Journal of Virology 29:698–704
    [Google Scholar]
  19. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones - an investigation of genetic factors affecting cell competence. Virology 16:147–151
    [Google Scholar]
  20. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus-induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  21. Morse L. S., Pereira L., Roizman B., Schaffer P. A. 1978; Anatomy of herpes simplex virus (HSV) DNA. X. Mapping of viral genes by analysis of polypeptides and functions specified by HSV-1 x HSV-2 recombinants. Journal of Virology 26:389–410
    [Google Scholar]
  22. Moss H., Chartrand P., Timbury M. C., Hay J. 1979; A mutant of herpes simplex virus type 2 with temperature-sensitive lesions affecting virion thermostability and deoxyribonuclease activity: identification of the lethal mutation and physical mapping of the nuc lesion. Journal of Virology 32:140–146
    [Google Scholar]
  23. Müller W., Weber H., Meyer F., Weissman C. 1978; Site directed mutagenesis in DNA: generation of point mutations in cloned B globin complementary DNA at the positions corresponding to amino acids 121-123. Journal of Molecular Biology 124:343–358
    [Google Scholar]
  24. Parris D. S., Dixon R. A. F., Schaffer P. A. 1980; Physical mapping of herpes simplex virus type 1 ts mutants by marker rescue: correlation of the physical and genetic maps. Virology 100:275–287
    [Google Scholar]
  25. Preston V. G., Davison A. J., Marsden H. S., Timbury M. C., Subak-Sharpe J. H., Wilkie N. M. 1978; Recombinants between herpes simplex virus types 1 and 2: analysis of genome structures and expression of immediate early polypeptides. Journal of Virology 28:499–517
    [Google Scholar]
  26. Ruyechan W. T., Morse L. S., Knipe D. M., Roizman B. 1979; Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behaviour of infected cells. Journal of Virology 29:677–697
    [Google Scholar]
  27. Schaffer P. A., Carter V. C., Timbury M. C. 1978; Collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2. Journal of Virology 27:490–504
    [Google Scholar]
  28. Sheldrick P., Berthelot N. 1974; Inverted repetition in the chromosome of herpes simplex virus. Cold Spring Harbor Symposia on Quantitative Biology 39:667–678
    [Google Scholar]
  29. Shortle D., Nathans D. 1978; Local mutagenesis: a method for generating viral mutants with fake substitutions in pre-selected regions of the genome. Proceedings of the National Academy of Sciences of the United States of America 75:2170–2174
    [Google Scholar]
  30. Skare J., Summers W. C. 1977; Structure and function of herpes virus genomes. II. Eco RI, Xba I, and Hind III endonuclease cleavage sites on herpes simplex virus type 1 DNA. Virology 76:581–595
    [Google Scholar]
  31. Stow N. D. 1978; Physical mapping of herpes simplex virus type 1 mutations. Ph.D. thesis University of Glasgow;
    [Google Scholar]
  32. Stow N. D., Wilkie N. M. 1976; An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. Journal of General Virology 33:447–458
    [Google Scholar]
  33. Stow N. D., Wilkie N. M. 1978; Physical mapping of temperature-sensitive mutations of herpes simplex virus type 1 by intertypic marker rescue. Virology 90:1–11
    [Google Scholar]
  34. Stow N. D., Subak-Sharpe J. H., Wilkie N. M. 1978; Physical mapping of herpes simplex virus type 1 mutations by marker rescue. Journal of Virology 28:182–192
    [Google Scholar]
  35. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type II. Journal of General Virology 13:373–376
    [Google Scholar]
  36. Timbury M. C., Calder L. 1976; Temperature-sensitive mutants of herpes simplex virus type 2: a provisional linkage map based on recombination analysis. Journal of General Virology 30:179–186
    [Google Scholar]
  37. Timbury M. C., Subak-Sharpe J. H. 1973; Genetic interactions between temperature-sensitive mutants of types 1 and 2 herpes simplex viruses. Journal of General Virology 18:347–357
    [Google Scholar]
  38. Wilkie N. M. 1973; The synthesis and substructure of herpes virus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA. Journal of General Virology 21:453–467
    [Google Scholar]
  39. Wilkie N. M. 1976; Physical maps for herpes simplex virus type 1 DNA for restriction endonucleases Hind III, Hpa I and X.bad. Journal of Virology 20:222–233
    [Google Scholar]
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