1887

Abstract

SUMMARY

The effect of human fibroblast interferon (IFN), a heterologous IFN, on the uncoating of murine leukaemia virus (MLV) was investigated in exogenously infected NIH/3T3 mouse cells. Virus uncoating was determined by following the disappearance of the penetrating virus particles from the cytoplasm of the infected cells. Intracellular virus particles were estimated by sedimenting them at high speed from the cytoplasmic fraction of the infected cells and assaying their reverse transcriptase activity. In untreated control cells, uncoating started immediately after penetration, but in cells treated with human IFN, uncoating was delayed for 2 to 3 h. This delay led to prolongation of the infectious cycle, with delayed release of progeny virus. The delay in release did not result from inhibition by IFN of the process of release, since in NIH/3T3 cells chronically infected with MLV, treatment with IFN had no effect on virus release.

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1980-12-01
2024-04-16
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References

  1. Aboud M., Weiss O., Salzberg S. 1976; A rapid quantitation of interferon with chronically oncornavirus-producing cells. Infection and Immunity 13:1626–1632
    [Google Scholar]
  2. Aboud M., Shoor R., Salzberg S. 1979; Adsorption penetration and uncoating of murine leukemia virus studied by using its reverse transcriptase. Journal of Virology 30:32–37
    [Google Scholar]
  3. Aboud M., Shoor R., Salzberg S. 1980; An effect of interferon on the uncoating of murine leukaemia virus not related to the antiviral state. Journal of General Virology 51:425–429
    [Google Scholar]
  4. Besanqon F., Ankel H. 1977; Membrane receptors for interferon. Texas Reports on Biology and Medicine 35:282–292
    [Google Scholar]
  5. Billiau A. 1977; Effect of interferon on RNA tumor viruses. Texas Reports on Biology and Medicine 35:406–419
    [Google Scholar]
  6. Chang E. H., Jay F. T., Friedman R. M. 1978; Physical, morphological and biochemical alterations in the membrane of AKR mouse cells after interferon treatment. Proceedings of the National Academy of Sciences of the United States of America 75:1859–1863
    [Google Scholar]
  7. Chany C. 1976; Membrane bound interferon specific cell receptor system: role in establishment of amplification of the antiviral state. Biomedicine 24:148–157
    [Google Scholar]
  8. Friedman R. M. 1977; Antiviral activity of interferons. Bacteriological Reviews 41:543–567
    [Google Scholar]
  9. Friedman R. M. 1978; Interferon action and the cell surface. Pharmacology and Therapeutics A 2:425–438
    [Google Scholar]
  10. Knight E. Jr, Korant B. D. 1977; A surface alteration in mouse L-cells induced by interferon. Biochemical and Biophysical Research Communications 74:707–713
    [Google Scholar]
  11. Lindahl P., Leary P., Gresser L. 1974; Enhancement of the expression of histocompatibility antigens of mouse lymphoid cells by interferon in vitro. European Journal of Immunology 4:779–784
    [Google Scholar]
  12. Pitha P. M., Wivel N. A., Fernie B. F., Harper H. P. 1979; Effect of interferon on murine leukaemia virus infection. IV. Formation of non-infectious virus in chronically infected cells. Journal of General Virology 42:467–480
    [Google Scholar]
  13. Salzberg S., Bakhanashvili M., Aboud M. 1978; Effect of interferon on mouse cells chronically infected with murine leukemia virus. Kinetic studies on virus production and viral RNA synthesis. Journal of General Virology 40:121–130
    [Google Scholar]
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