The double-stranded DNA of an adenovirus of tupaia (TAV) which has a mol. wt. of 21.5 × 10 was analysed as follows: the cleavage sites of restriction endonucleases HI, RI, I, I, EII, I and I were determined by complete, partial and double digestion followed by gel electrophoretic separation of the resulting fragments. Terminal I fragments were determined by hydrolysing the intact DNA with exonuclease III before restriction enzyme cleavage. Partial denaturation mapping of uncleaved DNA and RI fragments revealed the cleavage sites of RI as well as A+T-rich regions at both termini of the genome.


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