@article{mbs:/content/journal/jgv/10.1099/0022-1317-51-2-385, author = "Walker, D. P. and Hill, B. J.", title = "Studies on the Culture, Assay of Infectivity and Some in vitro Properties of Lymphocystis Virus", journal= "Journal of General Virology", year = "1980", volume = "51", number = "2", pages = "385-395", doi = "https://doi.org/10.1099/0022-1317-51-2-385", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-51-2-385", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY To facilitate an in vitro study of bluegill lymphocystis virus, a new cell line was established from the fins and caudal tissue of bluegill sunfish and designated BF-W. Bluegill lymphocystis virus was accurately and reproducibly assayed by an enlarged-cell enumeration assay and was propagated in BF-W cell cultures to give maximum titres of about 106 cell-enlarging units/ml in 21 days at 25 °C. The infectivity titre of preparations of bluegill lymphocystis virus was not affected by ultrasonic treatment, freezing and thawing, or long-term storage in maintenance medium at -70 or +4 °C, but decreased during storage at -20 °C in 50% glycerol. The infectivity of virus preparations was reduced by treatment with diethyl ether. Virus cultured in BF-W cells was entirely cell-associated and the growth of virus was completely inhibited by 1 mm-5-BrdUrd.", }