1887

Abstract

SUMMARY

To facilitate an study of bluegill lymphocystis virus, a new cell line was established from the fins and caudal tissue of bluegill sunfish and designated BF-W. Bluegill lymphocystis virus was accurately and reproducibly assayed by an enlarged-cell enumeration assay and was propagated in BF-W cell cultures to give maximum titres of about 10 cell-enlarging units/ml in 21 days at 25 °C. The infectivity titre of preparations of bluegill lymphocystis virus was not affected by ultrasonic treatment, freezing and thawing, or long-term storage in maintenance medium at -70 or +4 °C, but decreased during storage at -20 °C in 50% glycerol. The infectivity of virus preparations was reduced by treatment with diethyl ether. Virus cultured in BF-W cells was entirely cell-associated and the growth of virus was completely inhibited by 1 m-5-BrdUrd.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-51-2-385
1980-12-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/51/2/JV0510020385.html?itemId=/content/journal/jgv/10.1099/0022-1317-51-2-385&mimeType=html&fmt=ahah

References

  1. Black D. N., Brown F. 1976; Purification and physicochemical characteristics of African swine fever virus. Journal of General Virology 32:509–518
    [Google Scholar]
  2. Cooper P. D. 1967; The plaque assay of animal viruses. Methods in Virology 3:244–305
    [Google Scholar]
  3. Dulbecco R. 1952; The production of plaques in monolayer cultures by single particles of an animal virus. Proceedings of the National Academy of Sciences of the United States of America 38:747–752
    [Google Scholar]
  4. Dunbar C. E., Wolf K. 1966; The cytological course of experimental lymphocystis in the bluegill. Journal of Infectious Diseases 116:466–472
    [Google Scholar]
  5. Fenner F. 1976; The classification and nomenclature of viruses. Summary of the results of meetings of the International Committee on Taxonomy of Viruses in Madrid, September 1976. Journal of General Virology 31:463–470
    [Google Scholar]
  6. Hill B. J., Underwood B. O., Smale C. J., Brown F. 1975; Physico-chemical and serological characterization of five rhabdoviruses infecting fish. Journal of General Virology 27:369–378
    [Google Scholar]
  7. Hsu T. C. 1973; Karyology of cells in culture. In Tissue Culture Methods and Applications chapter 15: pp 764–767 Edited by Kruse P. F., Patterson M. K. New York and London: Academic Press;
    [Google Scholar]
  8. Karber G. 1931; Beitrag zur kollektiven behandlung pharmakologischer reinhenvirusche. Archiv für experimentelle Pathologie und Pharmakologie 162:480–487
    [Google Scholar]
  9. Kelly D. C., Robertson J. S. 1973; Icosahedral cytoplasmic deoxyriboviruses. Journal of General Virology 20:17–41
    [Google Scholar]
  10. Lopez D. M., Sigel M. M., Beasley A. R., Dietrich L. S. 1969; Biochemical and morphological studies of lymphocystis disease. National Cancer Institute Monograph No 31:223–236
    [Google Scholar]
  11. Midlige F. H., Malsberger R. G. 1968; In vitro morphology and maturation of lymphocystis virus. Journal of Virology 2:830–835
    [Google Scholar]
  12. Nigrelli R. F., Ruggieri G. D. 1965; Studies on virus diseases of fishes. Spontaneous and experimentally induced cellular hypertrophy (lymphocystis disease) in fishes of the New York aquarium, with a report of new cases and an annotated bibliography (1874–1965). Zoologica 50:83–96
    [Google Scholar]
  13. Nigrelli R. F., Smith G. M. 1939; Studies on lymphocystis disease in the orange filefish, Ceratacanthus schoepfi (Walbaum) from Sandy Hook Bay, New Jersey. Zoologica 24:255–264
    [Google Scholar]
  14. Pritchard H. N., Malsberger R. G. 1968; A cytochemical study of lymphocystis tumour cells in vivo. Journal of Experimental Zoology 169:371–380
    [Google Scholar]
  15. Shannon J. E. 1972 Registry of Animal Cell Lines Edited by Shannon J. E., Lacy M. L. The American Type Culture Collection, Rockville; Maryland, U. S. A.:
    [Google Scholar]
  16. Spurr A. R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 37:219–239
    [Google Scholar]
  17. Templeman W. 1965; Lymphocystis disease in American plaice of the Eastern Grand Bank. Journal of the Fisheries Research Board of Canada 22:1345–1355
    [Google Scholar]
  18. Teorrel T., Stenhagen E. 1938; Ein unwersal puffer für den pH-bereich 2, 0 bis 12, 0. Biochemische Zeit-schrift 299:416–419
    [Google Scholar]
  19. Underwood B. O., Smale C. J., Brown F., Hill B. J. 1977; Relationship of a virus from Tellina tenuis to infectious pancreatic necrosis virus. Journal of General Virology 36:93–109
    [Google Scholar]
  20. Walker R. 1965; Viral DNA and cytoplasmic RNA in lymphocystis cells. Annals of the New York Academy of Sciences 126:386–395
    [Google Scholar]
  21. Wharton F. H., Ellender R. D., Stocks P. K. 1974; In vitro cultivation of cells from the silver perch (Bairdiella chrysura): a substrate for lymphocystis replication. In Gulf Coastal Regional Symposium on Diseases of Aquatic Animals pp 143–151 Edited by Amborski R. L., Hood M. A., Millet R. R. Barton Rouge: The Louisiana State University, Publication Office;
    [Google Scholar]
  22. Wolf K. 1962; Experimental propagation of lymphocystis disease of fishes. Virology 18:249–256
    [Google Scholar]
  23. Wolf K. 1966; The fish viruses. Advances in Virus Research 12:36–98
    [Google Scholar]
  24. Wolf K., Carlson C. P. 1965; Multiplication of lymphocystis virus in the bluegill (Lepomis macrochirus). Annals of the New York Academy of Sciences 126:414–419
    [Google Scholar]
  25. Wolf K., Quimby M. C. 1973; Fish viruses: buffers and methods for plaquing eight agents under normal atmosphere. Applied Microbiology 25:659–664
    [Google Scholar]
  26. Wolf K., Gravell M., Malsberger R. G. 1966; Lymphocystis virus: isolation and propagation in centrarchid fish cell lines. Science 151:1004–1005
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-51-2-385
Loading
/content/journal/jgv/10.1099/0022-1317-51-2-385
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error