%0 Journal Article %A Rottier, Peter J. M. %A Rezelman, Geertje %A Van Kammen, Ab %T Protein Synthesis in Cowpea Mosaic Virus-infected Cowpea Protoplasts: Further Characterization of Virus-related Protein Synthesis %D 1980 %J Journal of General Virology, %V 51 %N 2 %P 373-383 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-51-2-373 %I Microbiology Society, %X SUMMARY Inoculation of cowpea protoplasts with purified middle component of cowpea mosaic virus (CPMV) did not result in the production of newly induced virus-related proteins. In contrast, infection of protoplasts with purified bottom component produced virus-related polypeptides with mol. wt. 170×103, 112 × 103, 110 × 103, 84 × 103 and 30 × 103, but not the virus capsid proteins. These results suggest that CPMV B-RNA specifies one or more early functions, one of which is the replicase function, while m-RNA encodes the virus coat proteins. Attempts to elucidate the mechanism by which the virus-specific proteins are synthesized were inconclusive. No precursor-product relations could be demonstrated and virus subgenomic messengers were not observed. Some kind of processing was nonetheless found to occur with the small virus capsid protein:several discrete oligopeptides were successively cleaved from this protein after assembly into the virion particle. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-51-2-373