1887

Journal of General Virology header logo

Volume 51, Issue 2

Protein Synthesis in Cowpea Mosaic Virus-infected Cowpea Protoplasts: Detection of Virus-related Proteins Free

Abstract

SUMMARY

A study was made of the proteins synthesized in cowpea protoplasts infected with cowpea mosaic virus (CPMV). Protoplasts were labelled with S-methionine at different times during the infection cycle and the proteins synthesized were analysed by SDS-polyacrylamide slab gel electrophoresis of different subcellular fractions. Since no shut-off of host protein synthesis occurred, virus-specific protein synthesis had to be distinguished from a high background of protoplast proteins. Eleven polypeptides with mol. wt. of 170, 130, 112, 110, 87, 84, 68, 37, 30, 24 and 23 (all × 10) were detected whose synthesis was either induced or stimulated by CPMV infection. No differences were observed between the electrophoretic patterns of samples from uninfected and CPMV-infected protoplasts during the first 8 h of infection (latent phase). The 170 × 10 and 30 × 10 species were the first virus-related polypeptides detectable by labelling between 9 and 15 h after inoculation. From about 16 h after inoculation, all other virus-related proteins appeared to be synthesized at increasing rates. The polypeptides with mol. wt. of 170, 130, 112, 110, 68, 37 and 23 (all × 10) could be designated as virus-induced; the others may well be host-coded proteins, the synthesis of which is stimulated by CPMV infection. Although CPMV replication is known to occur in close association with virus-induced cytopathic membrane structures the 112 × 10 and 68 × 10 mol. wt. polypeptides were the only virus-related proteins specifically found in the particulate fractions of the protoplasts. From the 11 virus-related proteins, the 37 × 10, 24 × 10 and 23 × 10 mol. wt. proteins were identified as virus coat proteins. The 170 × 10 and 30 × 10 mol. wt. polypeptides also appeared to be virus-specific and to be coded for by CPMV bottom component RNA.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1980
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-51-2-359
1980-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/51/2/JV0510020359.html?itemId=/content/journal/jgv/10.1099/0022-1317-51-2-359&mimeType=html&fmt=ahah

References

  1. Carrasco L. 1977; The inhibition of cell functions after viral infection. A proposed general mechanism. FEBS Letters 76:11–15
     [Google Scholar]
  2. DE Zoeten O. A., Assink A. M., Van Kammen A. 1974; Association of cowpea mosaic virus-induced doublestranded RNA with a cytopathological structure in infected cells. Virology 59:341–355
     [Google Scholar]
  3. Ellis R. J. 1977; Protein synthesis by isolated chloroplasts. Biochimica et Biophysica Acta 463:185–215
     [Google Scholar]
  4. Hibi T., Rezelman G., van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
     [Google Scholar]
  5. Kedinger C., Gissinger F., Chambon P. 1974; Animal DNA-dependent RNA polymerases. Molecular structures and immunological properties of calf-thymus enzyme AI and calf-thymus and rat-liver enzymes B. European Journal of Biochemistry 44:421–436
     [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
     [Google Scholar]
  7. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpesvirus-infected cells: analysis of the polypeptides induced by wild type and sixteen temperature-sensitive mutants of HSV strain 17. Journal of General Virology 31:347–372
     [Google Scholar]
  8. Okuno T., Furusawa I. 1979; RNA polymerase activity and protein synthesis in brome mosaic virus-infected protoplasts. Virology 99:218–225
     [Google Scholar]
  9. Paterson R., Knight C. A. 1975; Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus. Virology 64:10–22
     [Google Scholar]
  10. Pelham H. R. B. 1979; Synthesis and proteolytic processing of cowpea mosaic virus proteins in reticulocyte lysates. Virology 96:463–477
     [Google Scholar]
  11. Rottier P. J. M., Rezelman G., Van Kammen A. 1979; The inhibition of cowpea mosaic virus replication by actinomycin D. Virology 92:299–309
     [Google Scholar]
  12. Sakai F., Takebe I. 1974; Protein synthesis in tobacco mesophyll protoplasts induced by tobacco mosaic virus infection. Virology 62:426–433
     [Google Scholar]
  13. Sakai F., Watts J. W., Dawson J. R. O., Bancroft J. B. 1977; Synthesis of proteins in tobacco protoplasts infected with cowpea chlorotic mottle virus. Journal of General Virology 34:285–293
     [Google Scholar]
  14. Sakai F., Dawson J. R. O., Watts J. W. 1979; Synthesis of proteins in tobacco protoplasts infected with brome mosaic virus. Journal of General Virology 42:323–328
     [Google Scholar]
  15. Siegel A., Hari V., Kolacz K. 1978; The effect of tobacco mosaic virus infection on host and virus-specific protein synthesis in protoplasts. Virology 85:494–503
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-51-2-359
Loading
Protein Synthesis in Cowpea Mosaic Virus-infected Cowpea Protoplasts: Detection of Virus-related Proteins
J. Gen. Virol. 51, 359 (1980); https://doi.org/10.1099/0022-1317-51-2-359
/content/journal/jgv/10.1099/0022-1317-51-2-359
/content/journal/jgv/10.1099/0022-1317-51-2-359
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error