1887

Abstract

SUMMARY

RNA from the Snyder–Theilen feline sarcoma–leukaemia virus complex (ST-FeSV–FeLV) sedimented in a double-peaked band between 50 and 70S, but Gardner-Arnstein (GA) FeSV–FeLV RNA sedimented in a single 70S peak. FeLV isolated from the ST virus mixture contained RNA which sedimented in a 70S band like GA-FeSV–FeLV RNA, but F422 FeLV RNA sedimented more slowly, at 50 to 60S. After thermal denaturation, resedimentation revealed three classes of RNA subunits in ST-FeSV–FeLV RNA: the first class, 35 to 37S, was also found in ST-FeLV and other FeLVs (except F422 FeLV), in the endogenous feline virus, RD114 and in GA-FeSV–FeLV; the second class, 32 to 34S, was similar to subunits in F422 FeLV and minor components of GA-FeSV–FeLV and ST-FeLV; the third class, 25S, was detected only in ST-FeSV–FeLV RNA. Electrophoresis of RNA species in buffered formamide provided evidence that the three classes of RNA subunits distinguishable on the basis of sedimentation rates actually represent three size classes of subunits. The ST virus mixture was shown to contain about equal titres of infectious FeLV and transforming FeSV whereas GA-FeSV–FeLV had at least a 10-fold excess of FeLV over FeSV. These observations are discussed in terms of possible origins of the three sizes of FeSV–FeLV RNA subunits and their relationships to three species of FeSV–FeLV proviral DNA described recently (Sherr ., 1979).

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1980-12-01
2024-03-29
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References

  1. Besmer P., Olshevsky V., Baltimore D., Dolberg D., Fan H. 1979; Virus-like 30S RNA in mouse cells. Journal of Virology 29:1168–1176
    [Google Scholar]
  2. Brian D. A., Thomason A. R., Rottman F. M., Velicer L. F. 1975; Properties of feline leukemia virus.III. Analysis of the RNA. Journal of Virology 16:535–545
    [Google Scholar]
  3. Conley A. J., Velicer L. F. 1978; Analysis of cytoplasmic RNA and polyribosomes from feline leukemia virus-infected cells. Journal of Virology 25:750–763
    [Google Scholar]
  4. Duc-Nguyen H., Rosenblum E. N., Zeigel R. F. 1966; Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. Journal of Bacteriology 92:1133–1140
    [Google Scholar]
  5. Duesberg P. H., Scolnick E. H. 1977; Murine type C viruses propagated in mouse cells contain a 30S RNA subunit of unknown biological activity in addition to the 38S subunit of the viral genome. Virology 33:211–216
    [Google Scholar]
  6. Duesberg P. H., Vogt P. K. 1973; Gel electrophoresis of avian leukosis and sarcoma viral RNA in formamide: comparison with other viral and cellular RNA species. Journal of Virology 12:594–599
    [Google Scholar]
  7. Erikson R. 1969; Studies on RNA from avian myeloblastosis virus. Virology 34:124–131
    [Google Scholar]
  8. Essex M. 1975; Horizontally and vertically transmitted oncornaviruses of cats. Advances in Cancer Research 21:175–248
    [Google Scholar]
  9. Gardner M. B., Rongey R. W., Arnstein P., Ested J. D., Sarma P., Huebner R. J., Rickard C. G. 1970; Experimental transmission of feline fibrosarcoma to cats and dogs. Nature, London 226:807–812
    [Google Scholar]
  10. Hu S., Davidson N., Verma L. 1977; A heteroduplex study of the sequence relationships between the RNAs of m-MSV and m-MLV. Cell 10:469–477
    [Google Scholar]
  11. Kimball P. C., Michalides R., Schlom J. 1976; Characterizations of mouse mammary tumor viruses from primary tumor cell cultures. II. Biochemical and biophysical studies. Journal of the National Cancer Institute 56:119–124
    [Google Scholar]
  12. Kimball P. C., Simon M. C., Mishra N. K. 1980; Toward a model for the molecular genetics of carcinogenesis in rats. Proceedings of the National Academy of Sciences of the United States of America 77:3750–3757
    [Google Scholar]
  13. Kirsten W. H., Mayer L. A. 1967; Morphological responses to a murine erythroblastosis virus. Journal of the National Cancer Institute 39:311–335
    [Google Scholar]
  14. Lehrach H., Diamond D., Wozney J. M., Boedtker H. 1977; RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751
    [Google Scholar]
  15. McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V., Kabigting A., Vernon L. 1972; C-type virus released from cultured human rhabdomyosarcoma cells. Nature New Biology 235:36–39
    [Google Scholar]
  16. Maisel J., Klement V., Lai M. M.-C., Ostertag W., Duesberg P. H. 1973; Ribonucleic acid components of murine sarcoma and leukemia viruses. Proceedings of the National Academy of Sciences of the United States of America 70:3536–3540
    [Google Scholar]
  17. Maisel L., Dina D., Duesberg P. H. 1977; Murine sarcoma viruses: the helper-independence reported for a Moloney variant is unconfirmed; distinct strains differ in size of their RNAs. Virology 76:295–312
    [Google Scholar]
  18. Porzig K. J., Barbacid M., Aaronson S. A. 1979a; Biological properties and translational products of three independent isolates of feline sarcoma virus. Virology 92:91–107
    [Google Scholar]
  19. Porzig K. J., Robbins K. C., Aaronson S. A. 1979b; Cellular regulation of mammalian sarcoma virus expression: a gene regulation model for oncogenesis. Cell 16:875–884
    [Google Scholar]
  20. Rickard C. G., Post J. E., Noronha F., Barr L. M. 1969; A transmissible virus-induced lymphocytic leukemia of the cat. Journal of the National Cancer Institute 42:987–1014
    [Google Scholar]
  21. Sarma P. S., Log T. 1973; Subgroup classification of feline leukemia and sarcoma viruses by viral interference and neutralization tests. Virology 54:160–169
    [Google Scholar]
  22. Sherr C. J., Fedele L. A., Donner L., Turek L. P. 1979; Restriction endonuclease mapping of unintegrated proviral DNA of Snyder-Theilen feline sarcoma virus: localization of sarcoma-specific sequences. Journal of Virology 32:860–875
    [Google Scholar]
  23. Shih T. Y., Young H. A., Coffin J. M., Scolnick E. M. 1978; Physical map of the Kirsten sarcoma virus genome as determined by fingerprinting RNase T1-resistant oligonucleotides. Journal of Virology 25:238–252
    [Google Scholar]
  24. Snyder S. P., Theilen G. H. 1969; Transmissible feline fibrosarcoma. Nature, London 221:1074–1076
    [Google Scholar]
  25. Strauss E. M., Simon M. C., Kimball P. C. 1980; Efficient production of mammalian RNA tumor viruses in serum-free medium allows rapid RNA subunit purification. Journal of Virological Methods 1:213–221
    [Google Scholar]
  26. Thomason A. R., Brian D. A., Velicer L. F., Rottman F. M. 1976; Methylation of high-molecular-weight subunit RNA of feline leukemia virus. Journal of Virology 20:123–132
    [Google Scholar]
  27. Thomason A. R., Friderich K. H., Velicer L. F., Rottman F. 1978; Presence of 5′-terminal cap structures in virus-specific RNA from feline leukemia virus-infected cells. Journal of Virology 26:226–235
    [Google Scholar]
  28. Whalley J. M. 1973; Size differences in the ribonucleic acids of feline leukaemia virus. Journal of General Virology 21:39–46
    [Google Scholar]
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