1887

Abstract

SUMMARY

The virus proteins, reverse transcriptase (RT) and p30, were found to increase with time in the subcellular fractions of lymphocytic tissue from either the thymus or spleen of AKR mice with spontaneous lymphocytic leukaemia. Significant levels of RT activity were first detected in the microsomal fractions of the two tissues at 15 and 20 weeks old, respectively. Although low amounts of p30 could be found in both tissues within the first week of life, the overall increase in the amount of p30 within each tissue followed much the same course as that shown by RT. In addition, a protein complex consisting of p30 and RT was first found in thymus and spleen lymphocytes of 15 and 20 week-old animals, respectively. The complex increased in amount in both organs as the animals aged, reaching a maximum level in 30 week-old mice. Repeated attempts to detect other virus proteins such as gp70 in association with the complex by immunological means were unsuccessful. The complex could not be found in lymphocytic tissue taken from younger animals or in ‘non-target’ organs, such as liver or kidney, of animals with leukaemia. In animals treated with antiviral IgG, which delayed the development of spontaneous leukaemia, the complex did not appear until much later in life (45 weeks) and then in considerably smaller amounts.

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1980-12-01
2024-03-28
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References

  1. Bandyopadhyay A. K. 1975; Purification of DNA polymerase from JLS-V9 cells. Archives of Biochemistry and Biophysics 166:72–83
    [Google Scholar]
  2. Bandyopadhyay A. K. 1977; Effect of Rauscher leukemia virus specific proteins on reverse transcriptase. Journal of Biological Chemistry 252:5883–5887
    [Google Scholar]
  3. Bandyopadhyay A. K., Levy C. C. 1978; Effect of RNA tumor virus specific protein P30 on reverse transcriptase: intraspecies and interspecies interaction between P30 and reverse transcriptase. Journal of Biological Chemistry 253:8285–8290
    [Google Scholar]
  4. Bandyopadhyay A. K., Levy C. C. 1979; Interaction between reverse transcriptase and P30 of ecotropic and xenotropic viruses. Proceedings of the Annual Meeting of the American Society of Microbiology 79:284
    [Google Scholar]
  5. Bandyopadhyay A. K., Deepak J., Bowling M., Fish D. C. 1976; Isolation of a multiprotein complex from Rauscher leukemia virus. Proceedings of the American Association of Cancer Research 17:161–171
    [Google Scholar]
  6. Benade L., Ihle J. N., Decleve A. 1978; Serological characterization of B-tropic viruses of C57/BL mice: possible origin by recombination of endogenous N- and X-tropic viruses. Proceedings of the National Academy of Sciences of the United States of America 74:4553–4557
    [Google Scholar]
  7. Biswal N., Benyesh-Melnick M. 1969; Complementary nuclear RNAs of murine sarcoma leukemia virus complex in transformed cells. Proceedings of the National Academy of Sciences of the United States of America 64:1371–1379
    [Google Scholar]
  8. Bollum F. J. 1967; Enzymatic synthesis of polydeoxynucleotides. In Genetic Elements, Properties and Function pp 3–15 Edited by Sugar D. New York: Academic Press;
    [Google Scholar]
  9. Chattopadhyay S. K., Lowy D. R., Teich N. M., Levine A. S., Rowe W. P. 1974; Evidence that AKR murine leukemia virus genome is complete in DNA of the high virus AKR mouse and incomplete in the DNA of the ‘virus negative’ NIH mouse. Proceedings of the National Academy of Sciences of the United States of America 71:167–171
    [Google Scholar]
  10. Deepak J., Comer J., Dobbs J., Bowling M., Aldenderfer P. H., Fish D. C., Bandyopadhyay A. K. 1975; Simultaneous purification of reverse transcriptase and gs-antigen from Rauscher leukemia virus. Biochemical and Biophysical Research Communications 63:400–408
    [Google Scholar]
  11. Elder T. H., Gautsch J. W., Jensen F. C., Lerner R. A., Hartley J. W., Rowe W. P. 1978; Biochemical evidence that MCF murine leukemia viruses are envelope (ENV) gene recombinants. Proceedings of the National Academy of Sciences of the United States of America 74:4676–4680
    [Google Scholar]
  12. Fish D. C., Gilden R. V., Bare R. M., Trimmer R. M., Huebner R. J. 1979; Complete prevention of spontaneous leukemia in AKR mice by passive immunization and type specificity of this protection. Journal of the National Cancer Institute 62:943–945
    [Google Scholar]
  13. Gross L. 1974 In Oncogenic Viruses 3rd edn pp 356–560 Edited by Gross L. New York: Pergamon Press;
    [Google Scholar]
  14. Hartley J. W., Wolford N. K., Old L. J., Rowe W. P. 1977; A new class of murine leukemia virus associated with the development of spontaneous lymphomas. Proceedings of the National Academy of Sciences of the United States of America 74:789–792
    [Google Scholar]
  15. Hass M. 1978; Leukemogenic activity of thymotropic, ecotropic and xenotropic radiation leukemia virus isolates. Journal of Virology 25:705–709
    [Google Scholar]
  16. Huebner R. J., Gilden R. V., Toni R., Hill P. W., Trimmer R. W., Fish D. C., Sass B. 1976; Prevention of spontaneous leukemia in AKR mice by type-specific immunosuppression of endogenous ecotropic virogene. Proceedings of the National Academy of Sciences of the United States of America 73:4633–4635
    [Google Scholar]
  17. Hunter W. M. 1967; Radioimmunoassay. In Handbook of Experimental Immunology pp 608–642 Edited by Weir D. M. Philadelphia: F. A. Davis;
    [Google Scholar]
  18. Jaffe E. S., Shevach E. M., Frank M. M., Berard C. W., Green I. 1974; Nodular lymphoma-evidence for origin from follicular β lymphocytes. New England Journal of Medicine 290:813–818
    [Google Scholar]
  19. Jamjoom G. A., Naso R. B., Arlinghaus R. B. 1977; Further characterization of int0racellular polyprotein precursors of Rauscher leukemia virus. Virology 78:11–34
    [Google Scholar]
  20. Kwaeshima J., Ikeda H., Hartley J. W., Stockert E., Rowe W. P., Old L. J. 1976; Changes in expression of murine leukemia virus antigen and production of xenotropic virus in late preleukemic period of AKR mice. Proceedings of the National Academy of Sciences of the United States of America 73:4680–4684
    [Google Scholar]
  21. Levy J. 1973; Xenotropic viruses: murine leukemia associated with NIH swiss, NZB and other mouse strains. Science 182:1151–1153
    [Google Scholar]
  22. Metcalf D. 1966; The thymus. In Recent Results in Cancer Research vol 5: pp 1–128 Edited by Metcalf D. New York: Springer-Verlag;
    [Google Scholar]
  23. Nowinskt R. C., Doyle T. 1977; Cellular changes in the thymuses of preleukemic AKR mice: correlation of changes in the expression of murine leukemia viruses. Cell 12:341–353
    [Google Scholar]
  24. Rauscher F. J. 1962; A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. Journal of the National Cancer Institute 29:515–532
    [Google Scholar]
  25. Rowe W. P. 1972; Studies of genetic transmission of murine leukemia viruses by AKR mice. I. Crosses with Fv-I in strains of mice. Journal of Experimental Medicine 136:1272–1285
    [Google Scholar]
  26. Rowe W. P., Pincus T. 1972; Quantitative studies of naturally occurring murine leukemia infection of AKR mice. Journal of Experimental Medicine 135:429–436
    [Google Scholar]
  27. Strand M., August J. T. 1973; Structural proteins of oncogenic ribonucleic acid viruses: interspec II, a new interspecies antigen. Journal of Biological Chemistry 288:5627–5633
    [Google Scholar]
  28. Swanson S. K., Sulkowski E., Manly K. 1978; Hydrophobic binding site(s) on Moloney-murine leukemia virus P30. Virology 85:21–221
    [Google Scholar]
  29. Todaro G. J., Arnstein P., Parks W. P., Lennette E. H., Huebner R. J. 1973; A type C virus in human Rhabdomyosarcoma cells after inoculation into NIH swiss mice treated with antithymocyte serum. Proceedings of the National Academy of Sciences of the United States of America 70:859–862
    [Google Scholar]
  30. Troxler D. H., Lowy D., Howk R., Young H., Scolnick E. M. 1977; Friend strain of spleen-focus forming virus: a recombinant between ecotropic murine type C and the envelope gene region of xenotropic type C virus. Proceedings of the National Academy of Sciences of the United States of America 74:4671–4675
    [Google Scholar]
  31. Weissbach A. 1977; Eukaryotic DNA polymerases. Annual Review of Biochemistry 46:25–49
    [Google Scholar]
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