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It was shown that resistance of the cucumber cultivar China (Kyoto) to cucumber mosaic virus (CMV) functions at the single cell level. Measurement of physiological parameters has excluded the possibility that this result arises from the artificial procedures used in protoplast isolation and inoculation. The maintenance of resistance in protoplasts of China (Kyoto) after inoculation with CMV RNA indicates that the resistance determinant(s) most probably operate at transcription or translation stages.
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