1887

Abstract

SUMMARY

A mammalian cell-adapted mutant of the Dobson strain of fowl plague virus (FPV-B) was characterized. Genetic analyses of recombinants between a mutant of this virus and either the non-adapted Dobson strain or the Rostock strain of FPV showed that the gene coding for the P3 protein of the adapted Dobson strain was sufficient to enable any recombinant to grow in L cells.

The abortive cycle of wild-type Dobson strain (FPV+) was compared to the productive cycle of the mutant. By using 100 p.f.u./cell, no quantitative difference could be detected in infected L cells between polypeptides and cRNAs induced by FPV+ and FPV-B. However, the maturation of virions at the plasma membrane did not proceed correctly. At a lower m.o.i. the amounts of virus polypeptides decreased with the m.o.i. This decrease was not the same for all polypeptides and cRNA segments: HA, M and NA and their mRNAs decreased to a greater extent than the others. These results are discussed in relation to a possible biological activity of polypeptide P3.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-51-1-33
1980-11-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/51/1/JV0510010033.html?itemId=/content/journal/jgv/10.1099/0022-1317-51-1-33&mimeType=html&fmt=ahah

References

  1. Almond J. W. 1977; A single gene determines the host range of influenza. Nature, London 270:617–618
    [Google Scholar]
  2. Almond J. W., Barry R. D. 1979; Genetic recombination between two strains of fowl plague virus: construction of genetic maps. Virology 92:407–415
    [Google Scholar]
  3. Avery R. J. 1975; Abortive infection of L cells by influenza virus: absence of virion RNA synthesis. Journal of Virology 16:311–314
    [Google Scholar]
  4. Bosch F. X., Hay A. J., Skehel J. J. 1978; RNA and protein synthesis in a permissive and an abortive influenza virus infection. In Negative Strand Viruses and the Host Cell pp 465–474 Edited by Mahy B. W. J., Barry R. D. New York and London: Academic Press;
    [Google Scholar]
  5. Caliguiri L. A., Holmes K. V. 1979; Host-dependent restriction of influenza virus maturation. Virology 92:15–30
    [Google Scholar]
  6. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in SDS and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  7. Franklin R. M., Breitenfeld P. M. 1959; The abortive infection of Earle’s L cells by fowl plague virus. Virology 8:293–307
    [Google Scholar]
  8. Fraser K. B. 1967; Immunofluorescence of abortive and complete infection by influenza A virus in hamster BHK21 cells and mouse L cells. Journal of General Virology 1:1–12
    [Google Scholar]
  9. Gandhi S. S., Bell H. B., Burke D. C. 1971; Abortive infection of L cells by fowl plague virus: comparison of RNA and protein synthesis in infected chick and L cells. Journal of General Virology 13:423–432
    [Google Scholar]
  10. Hay A. J., Bellamy A. R., Abraham G., Skehel J. J., Brand C. M., Webster R. G. 1977a; Procedures, for the characterization of the genetic material of candidate vaccine strains. In International Symposium on Influenza Immunization, vol. II, Geneva, 1977. Developments in Biological Standardization 39:15–24
    [Google Scholar]
  11. Hay A. J., Lomniczi B., Bellamy A. R., Skehel J. J. 1977b; Transcription of the influenza virus genome. Virology 83:337–355
    [Google Scholar]
  12. Hay A. J., Skehel J. J., Webster R. G. 1979; Differentiation of the haemagglutinin genes of variant influenza viruses by RNA-RNA hybridization. Journal of General Virology 45:245–248
    [Google Scholar]
  13. Inglis S. C., Mahy B. W. J. 1979; Polypeptides specified by the influenza virus genome. 3. Control of synthesis in infected cells. Virology 95:154–164
    [Google Scholar]
  14. Inglis S. C., Mcgeogh D. J., Mahy B. W. J. 1976; Polypeptides specified by the influenza virus genome. I. Evidence for eight distinct gene products specified by fowl plague virus. Virology 74:489–503
    [Google Scholar]
  15. Israël A. 1979; Preliminary characterization of the particles from productive and abortive infections of L cells by Fowl plague virus. Annales de Microbiologie 130B:85–100
    [Google Scholar]
  16. Israël A. 1980a; Productive and abortive infection of L cells by fowl plague virus (FPV): comparison of in vivo and in vitro translation products of the viral mRNAs. Journal of General Virology 47:473–483
    [Google Scholar]
  17. Israël A. 1980b; A temperature-dependent host-range mutant of fowl plague virus (FPV). Virology (in press)
    [Google Scholar]
  18. Israël A., Semmel M., Huppert J. 1975; Host-range of fowl plague virus (FPV): comparison of the genome and virus proteins. Virology 68:503–509
    [Google Scholar]
  19. Israël A., Niveleau A., Quash G., Richard M. H. 1979; Latex fetuin spheres as probes for influenza virus neuraminidase in productively and abortively infected cells. Archives of Virology 61:183–199
    [Google Scholar]
  20. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  21. Meier-Ewert H., Compans R. W. 1974; Time-course of synthesis and assembly of influenza virus proteins. Journal of Virology 14:1083–1091
    [Google Scholar]
  22. Scholtissek C., Murphy B. R. 1978; Host-range mutants of an influenza A virus. Archives of Virology 58:323–333
    [Google Scholar]
  23. Scholtissek C., Rott R. 1970; Synthesis in vivo of influenza virus plus and minus strand RNA and its preferential inhibition by antibiotics. Virology 40:989–996
    [Google Scholar]
  24. Skehel J. L. 1972; Polypeptide synthesis in influenza virus-infected cells. Virology 49:23–36
    [Google Scholar]
  25. Skehel J. J. 1973; Early polypeptide synthesis in influenza virus-infected cells. Virology 56:394–399
    [Google Scholar]
  26. Zavada J. 1969; A host cell DNA function involved in the replication of avian tumor viruses and of FPV. Journal of General Virology 4571–579
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-51-1-33
Loading
/content/journal/jgv/10.1099/0022-1317-51-1-33
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error