1887

Abstract

SUMMARY

Human leucocyte interferon (HuLeIF) was purified by a series of techniques involving precipitation, gel filtration, Cu-chelate-, blue dextran- and antibody-affinity chromatography. The two major species of HuLeIF were identified in SDS-PAGE as two clearly separable and stainable proteins representing 85% of the biological activity. Three more species of HuLeIF representing 15% of the biological activity were also demonstrated. The specific activity of pure interferon proteins was approx. 10 IFU/mg protein. Recovery was about 50% and the purification factor exceeded 350000. All five species of HuLeIF had definite anticellular activities when tested with Daudi cells (inhibition of thymidine uptake).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-50-2-441
1980-10-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/50/2/JV0500020441.html?itemId=/content/journal/jgv/10.1099/0022-1317-50-2-441&mimeType=html&fmt=ahah

References

  1. Berg K. 1977; Sequential antibody affinity chromatography of human leukocyte interferon. Scandinavian Journal of Immunology 6:77–86
    [Google Scholar]
  2. Berg K., Heron I. 1980; The complete purification of human leukocyte interferon. Scandinavian Journal of Immunology n:489–502
    [Google Scholar]
  3. Berg K., Heron I., Hamilton R. 1978; Purification of human interferons by antibody affinity chromatography, using highly absorbed anti-interferon. Scandinavian Journal of Immunology 8:429–436
    [Google Scholar]
  4. Bollin E., Vastola K., Olezek D., Sulkowski E. 1978; The interaction of mammalian interferons with immobilized Cibacrom Blue F3GA: modulation of binding strength. Preparative Biochemistry 8:259–274
    [Google Scholar]
  5. Cantell K., Hirvonen S. 1978; Large-scale production of human leukocyte interferon containing 10s units per ml. Journal of General Virology 39:541–543
    [Google Scholar]
  6. Christophersen I. S., Jordan R., Osther K., Lindenberg J., Pedersen P. H., Berg K. 1978; Interferon therapy in neoplastic disease. Acta medica Scandinavica 204:471–476
    [Google Scholar]
  7. Dahl H. 1977; Differentiation between antiviral and anticellular effects of interferon. Texas Reports on Biology and Medicine 35:381–387
    [Google Scholar]
  8. Dahl H., Degre M. F. 1975; Separation of antiviral activity of human interferon from cell growth inhibitory effect. Nature, London 257:799–801
    [Google Scholar]
  9. Gordon A. H. 1969 In Electrophoresis of Proteins in Polyacrylamide and Starch Gels p 64 Amsterdam and London: North-Holland;
    [Google Scholar]
  10. Gresser L., Brouty-Boye D., Thomas M. T., Macieira-Coelho A. 1970; Interferon and cell division. I. Inhibition of the multiplication of mouse leukemia Li210 cells in vitro by interferon preparations. Proceedings of the National Academy of Sciences of the United States of America 66:1052–1058
    [Google Scholar]
  11. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. 1975; Two antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  12. Heron L., Berg K. 1978; The actions of interferon are potentiated at elevated temperature. Nature, London 274:508–510
    [Google Scholar]
  13. Hilfenhaus J., Damm H., Karges H. E., Manthey K. E. 1976; Growth inhibition of human lympho-blastoid Daudi cells in vitro by interferon preparations. Archives of Virology 51:87–97
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Lin L. S., Wiranowska-Stewart M., Chudzio T., Stewart W. E. II 1978; Characterization of the heterogeneous molecules of human interferon. Differences in the cross-species antiviral activities of various molecular populations in human leukocyte interferon. Journal of General Virology 39:125–130
    [Google Scholar]
  16. Morser J., Kabayo J. P., Hutchinson D. W. 1978; Differences in sialic content of human interferon. Journal of General Virology 41:175–178
    [Google Scholar]
  17. Paucker K., Cantell K., Henle W. 1962; Quantitative studies on viral interference in suspended L cells. III. Effect of interfering viruses and interferon on the growth rate of cells. Virology 17:324–334
    [Google Scholar]
  18. Porath J., Carlsson J., Olsson L., Belfrage G. 1975; Metal chelate affinity chromatography, a new approach to protein fractionation. Nature, London 258:598–599
    [Google Scholar]
  19. Rubinstein M., Rubinstein S., Familetti P. C., Gross M. S., Miller R. S., Waldman A. A., Pastka S. 1978; Human leukocyte interferon purified to homogeneity. Science 202:1289–1290
    [Google Scholar]
  20. Rubinstein M., Rubinstein S., Familletti P. C., Gross M. S., Miller R. S., Waldman A. A., Pestka S. 1978; Humna leukocyte interferon: production, purification to homogeneity and initial characterization. Proceedings of the National Academy of Sciences of the United States of America 76:640–644
    [Google Scholar]
  21. Stewart H W. E. 1979a In The Interferon System pp 179–183 Wien and New York: Springer-Verlag;
    [Google Scholar]
  22. Stewart H W. E. 1979b In The Interferon System p 238 Wien and New York: Springer-Verlag;
    [Google Scholar]
  23. Stewart H W. E., Desmyter J. 1975; Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation and cross-species antiviral activity. Virology 67:68–73
    [Google Scholar]
  24. Stewart H W. E., Gresser I., Tovey M. G., Bandu M.T., Legoff S. 1976; Identification of the cell multiplication inhibitory factors in interferon preparations as interferons. Nature, London 262:300–302
    [Google Scholar]
  25. Stewart H W. E., Lin L. S., Wiranowska-Stewart M., Cantell K. 1978; Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage. Proceedings of the National Academy of Sciences of the United States of America 74:4200–4204
    [Google Scholar]
  26. Törmä E., Paucker K. 1976; Purification and characterization of human leukocyte interferon components. Journal of Biological Chemistry 251:4810–4816
    [Google Scholar]
  27. Vilcek J., Havell E. A., Yamazaki S. 1976; Antigenic, physicochemical, and biological characterization of human interferon. Annals of the New York Academy of Sciences 284:703–710
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-2-441
Loading
/content/journal/jgv/10.1099/0022-1317-50-2-441
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error