1887

Abstract

SUMMARY

The tryptophan and tyrosine content of the bromelain-released subtype H3 haemagglutinin (H3 BHA) of influenza virus were measured by u.v. absorption and fluorescence techniques. The values obtained (8 and 18 residues, respectively) are in close agreement with those derived from amino acid analysis. Essentially all of the tryptophan residues are demonstrated to be localized on the surface of the BHA molecule.

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1980-10-01
2024-12-09
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References

  1. Brand C. M., Skehel J. J. 1972; Crystalline antigen from the influenza virus envelope. Nature New Biology 238:145–147
    [Google Scholar]
  2. Englander S. W., Epstein H. T. 1957; Optical methods for measuring nucleoprotein and nucleic acid concentrations. Archives of Biochemistry and Biophysics 68:144–149
    [Google Scholar]
  3. Flanagan M. T., Skehel J. J. 1977; The conformation of influenza virus haemagglutinin. FEES Letters 80:57–60
    [Google Scholar]
  4. Goodwin T. W., Morton R. A. 1946; The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochemical Journal 40:628–632
    [Google Scholar]
  5. Hartley B. S. 1964; Amino-acid sequence of bovine chymotrypsinogen-A. Nature, London 2or:1284–1287
    [Google Scholar]
  6. Ingham K. C., Tlonda C., Edelhoch H. 1976; Structural studies of human chorionic gonadotropin and its subunits using tyrosine fluorescence. Archives of Biochemistry and Biophysics 173:680–690
    [Google Scholar]
  7. Kharitonenkov L. G., Khristova M. L., Ruuge E. K. 1977; The investigation of the myxoviruses structure by spin probes method. I. Thermostability of the lipid membrane of influenza and Sendai viruses. Moleculjarnaja biologija II:432–439
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Lehrer S. S. 1971; Solute perturbation of protein fluorescence. The quenching of the tryptophyl fluorescence of model compounds and of lysozyme by iodide ion. Biochemistry 10:3254–3263
    [Google Scholar]
  10. Mikes O., Holeysovsky V., Tomassek V., Sorm F. 1966; Covalent structure of bovine trypsinogen. The position of the remaining amides. Biochemical and Biophysical Research Communications 24:346–352
    [Google Scholar]
  11. Pajot P. 1976; Fluorescence of proteins in 6 M-guanidine hydrochloride. A method for the quantitative determination of tryptophan. European Journal of Biochemistry 63:263–269
    [Google Scholar]
  12. Pelley R., Horowitz P. 1976; Fluorometric studies of tryptophyl exposure in concanavalin A. Bio-chimica et Biophysica Acta 427:359–363
    [Google Scholar]
  13. Schulze L. T. 1975; The biologically active proteins of influenza virus: the haemagglutinin. In Influenza Viruses and Influenza pp 53–82 Edited by Kilbourne E. D. New York, San Francisco, London: Academic Press;
    [Google Scholar]
  14. Shinitzky N., Rivnay B. 1977; Degree of exposure of membrane proteins determined by fluorescence quenching. Biochemistry 16:982–986
    [Google Scholar]
  15. Siniakov M. S., Kharitonenkov I. G., Arefiev I. M., Eskov A. P. 1980; Influenza virus haemagglutinin: hydrodynamic properties and molecular weight. Voprosy virusologii (USSR), No. 1 42–45
    [Google Scholar]
  16. Tristram G. R. 1953; The amino acid composition of proteins. In The Proteins 1 pp 181–233 Edited by Neurath H., Bailey K. New York: Academic Press;
    [Google Scholar]
  17. Vedenkina N. S., Burstein E. A. 1970; Tryptophan fluorescence of proteins in solution. Position of fluorescence spectral maxima. Moleculjarnaja biologija 4:743–748
    [Google Scholar]
  18. Ward C. W., Dopheide T. A. A. 1976; Size and chemical composition of influenza virus haemagglutinin chains. FEBS Letters 65:365–368
    [Google Scholar]
  19. Warren L. 1959; The thiobarbituric acid assay of sialic acids. Journal of Molecular Biology 234:1971–1975
    [Google Scholar]
  20. Wiley D. C., Skehel J. J., Waterfield M. 1977; Evidence from studies with a cross-linking reagent that the haemagglutinin of influenza virus is a trimer. Virology 79:446–448
    [Google Scholar]
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