The elderberry (E) and rhubarb (R) strains of cherry leaf roll virus were each purified and separated into middle (M) and bottom (B) nucleoprotein components by sucrose gradient centrifugation followed by near-equilibrium banding in caesium chloride. Heterologous mixtures of the RNAs from the separated components of the two strains were inoculated into a series of test plants. New local lesion types in thus induced were passaged three times through local lesions on to generate pure pseudorecombinant stocks. M-RNA determined serological specificity, the distribution of virus particle components, the type of systemic symptom in and cv. Samsun and Xanthi, and local and systemic symptoms in and B-RNA determined the ability to induce systemic symptoms in and the type of local lesion in . Backcrossing of the pseudorecombinants generated isolates with properties identical to those of the parental strains.


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