1887

Abstract

SUMMARY

Infectious particle production by temperature-sensitive () mutants of vesicular stomatitis virus (VSV) was measured in a variety of different host cell types maintained in a state of quiescence or stimulated to proliferate. At permissive temperatures, all mutants and the wild-type virus replicated equally well and with the same kinetics in both quiescent and proliferating cells. At semi-permissive temperatures, however, L mutants, with temperature-sensitive virion polymerases, showed a delay of about 6 h in infectious particle production relative to wild-type virus in proliferating cells and greater than 16 h in quiescent cells. The effect was specific for the L class of mutants and was not seen for representative mutants in any of the four other complementation groups of VSV. Regarding cellular determinants, the effect was correlated only with the growth phase and not with the species of origin, interferon inducibility or with malignant transformation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-50-2-337
1980-10-01
2022-11-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/50/2/JV0500020337.html?itemId=/content/journal/jgv/10.1099/0022-1317-50-2-337&mimeType=html&fmt=ahah

References

  1. Desmyter J., Melnick J. L., Rawls W. E. 1968; Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (VERO). Journal of Virology 2:955–961
    [Google Scholar]
  2. Farmilo A. J., Stanners C. P. 1972; Mutant of vesicular stomatitis virus which allows deoxyribonucleic acid synthesis and division in cells synthesizing viral ribonucleic acid. Journal of Virology 10:605–613
    [Google Scholar]
  3. Francoeur A. M., Lam T., Stanners C. P. 1980; PIF, a highly sensitive plaque assay for the induction of interferon. Virology (in the press)
    [Google Scholar]
  4. Holland J. T., Villarreal L. P. 1974; Persistent vesicular stomatitis virus infections mediated by defective T particles that suppress virion transcriptase. Proceedings of the National Academy of Sciences of the United States of America 71:2956–2960
    [Google Scholar]
  5. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  6. Pollard J. W., Stanners C. P. 1979; Characterization of cell lines showing growth control isolated from both the wild type and a leucyl-tRNA synthetase mutant of Chinese hamster ovary cells. Journal of Cellular Physiology 98:571–585
    [Google Scholar]
  7. Pringle C. R. 1978; The tdCE and hrCE phenotypes: host range mutants of vesicular stomatitis virus in which polymerase function is affected. Cell 15:597–606
    [Google Scholar]
  8. Sekellick M. J., Marcus P. I. 1978; Persistent infection. I. Interferon inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology 85:175–186
    [Google Scholar]
  9. Sekellick M. J., Marcus P. I. 1979; Persistent infection. II. Interferon inducing temperature-sensitive mutants as mediators of cell sparing: possible role in persistent infections by vesicular stomatitis virus. Virology 95:36–47
    [Google Scholar]
  10. Simpson P. W., Obdeski J. F., Morrangiello M. P. 1979; Conditional lethal mutants of vesicular stomatitis virus. III. Host range properties, interfering capacity, and complementation patterns of specific hr mutants. Virology 93:493–505
    [Google Scholar]
  11. Stanners C. P., Becker H. 1971; Control of macromolecular synthesis in proliferating and resting Syrian hamster cells in monolayer culture. I. Ribosome function. Journal of Cellular Physiology 77:31–42
    [Google Scholar]
  12. Stanners C. P., Goldberg V. J. 1975; On the mechanism of neurotropism of vesicular stomatitis virus in newborn hamsters. Studies with temperature-sensitive mutants. Journal of General Virology 29:281–296
    [Google Scholar]
  13. Stanners C. P., Lam T. 1978; The role of the tsh and P~ mutations of VSV T1026 in persistent infection. In Negative Strand Viruses and the Host Celt pp 577–582 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  14. Stanners C. P., Till J. E., Siminovitch L. 1963; Studies on the transformation of hamster embryo cells in culture by polyoma virus. I. Properties of transformed and normal cells. Virology 21:448–463
    [Google Scholar]
  15. Stanners C. P., Eliceiri G. L., Green H. 1971; Two types of ribosomes in mouse-hamster hybrid cells. Nature New Biology 230:52–54
    [Google Scholar]
  16. Stanners C. P., Francoeur A. M., Lam T. 1977; Analysis of a vesicular stomatitis virus mutant with attenuated cytopathogenicity: mutation in viral function, P, for inhibition of protein synthesis. Cell ir:272–281
    [Google Scholar]
  17. Stanners C. P., Wightman T. M., Harkins J. L. 1978; Effect of extreme amino acid starvation on the protein synthetic machinery of CHO cells. Journal of Cellular Physiology 95:125–238
    [Google Scholar]
  18. Stanners C. P., Adams M. E., Harkins J. L., Pollard J. W. 1979; Transformed cells have lost control of ribosome number through their growth cycle. Journal of Cellular Physiology 100:127–138
    [Google Scholar]
  19. Thompson L. H., Harkins J. L., Stanners C. P. 1973; A mammalian cell mutant with a temperature sensitive leucyl-tRNA synthetase. Proceedings of the National Academy of Sciences of the United States of America 70:3094–3098
    [Google Scholar]
  20. Youngner J. S., Dubovi E. J., Quagliana D. O., Kelly M., Preble O. T. 1976; Role of temperature sensitive mutants in persistent infection initiated with vesicular stomatitis virus. Journal of Virology 19:90–101
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-2-337
Loading
/content/journal/jgv/10.1099/0022-1317-50-2-337
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error