Two morphology mutants designated -5 and -6, of nuclear polyhedrosis virus (NPV) were isolated from virus grown in the presence of -methyl-′-nitro--nitrosoguanidine. Infected cells contained single, large cuboidal inclusion bodies with a crystalline lattice ultrastructure and envelope similar to that of wild-type (wt) polyhedra. The inclusion bodies had low infectivity when fed to larvae. The paracrystalline lattice structure of the mutants was similar to wt, but occluded viruses were rarely found within the mutants. The -5 polyhedrin (mol. wt. 30000) could be distinguished from wt and -6 polyhedrin on the basis of migration in SDS-PAGE gels. Peptide maps of polyhedrin were obtained following digestion with chymotrypsinogen or V8 protease. They were identical for -6 and wt polyhedrin but -5 polyhedrin gave a different pattern. Thus, the altered morphology may be due to a change in polyhedrin composition not detectable in -6 polyhedrin by the methods used here, or it may be the result of a mutation affecting a protein not yet identified.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error