1887

Abstract

SUMMARY

Two morphology mutants designated -5 and -6, of nuclear polyhedrosis virus (NPV) were isolated from virus grown in the presence of -methyl-′-nitro--nitrosoguanidine. Infected cells contained single, large cuboidal inclusion bodies with a crystalline lattice ultrastructure and envelope similar to that of wild-type (wt) polyhedra. The inclusion bodies had low infectivity when fed to larvae. The paracrystalline lattice structure of the mutants was similar to wt, but occluded viruses were rarely found within the mutants. The -5 polyhedrin (mol. wt. 30000) could be distinguished from wt and -6 polyhedrin on the basis of migration in SDS-PAGE gels. Peptide maps of polyhedrin were obtained following digestion with chymotrypsinogen or V8 protease. They were identical for -6 and wt polyhedrin but -5 polyhedrin gave a different pattern. Thus, the altered morphology may be due to a change in polyhedrin composition not detectable in -6 polyhedrin by the methods used here, or it may be the result of a mutation affecting a protein not yet identified.

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1980-10-01
2022-01-25
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References

  1. Bergold G. H. 1963a; The nature of nuclear polyhedrosis viruses. In Insect Pathology vol l pp 413–456 Edited by Steinhaus E. A. New York: Academic Press;
    [Google Scholar]
  2. Bergold G. H. 1963b; The molecular structure of some insect virus inclusion bodies. Journal of Ultrastructure Research 8:360–368
    [Google Scholar]
  3. Brown M., Faulkner P. 1975; Factors affecting the yield of virus in a cloned cell line of Trichoplusia ni infected with a nuclear polyhedrosis virus. Journal of Invertebrate Pathology 25:251–257
    [Google Scholar]
  4. Brown M., Faulkner P. 1978; Plaque assay of nuclear polyhedrosis viruses in cell culture. Applied Environmental Microbiology 36:31–35
    [Google Scholar]
  5. Brown M., Faulkner P. 1980; A partial genetic map of the baculovirus, Autographa californica nuclear polyhedrosis virus, based on recombination studies with ts mutants. Journal of General Virology 48:247–251
    [Google Scholar]
  6. Brown M., Crawford A. M., Faulkner P. 1979; Genetic analysis of a baculovirus, Autographa californica nuclear polyhedrosis virus, i. Isolation of temperature sensitive mutants and assortment into complementation groups. Journal of Virology 31:190–198
    [Google Scholar]
  7. Chung K. L., Brown M., Faulkner P. 1980; Studies on the morphogenesis of polyhedral inclusion bodies of a baculovirus Autographa californica NPV. Journal of General Virology 46:335–347
    [Google Scholar]
  8. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecylsulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  9. Dawson R. M. C., Elliott D. C., Elliott W. H., Jones K. M. 1969 In Data for Biochemical Research 2nd edn., pp 625–626 Oxford: Clarendon Press;
    [Google Scholar]
  10. Faulkner P., Henderson J. F. 1972; Serial passage of a nuclear polyhedrosis disease virus of the cabbage looper (Trichoplusia ni) in a continuous tissue culture cell line. Virology 50:920–924
    [Google Scholar]
  11. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of Lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
    [Google Scholar]
  12. Gershenson S. M. 1960; A study on a mutant strain of nuclear polyhedral virus of oak silkworm. Problems of Virology (English translation of Voprosy virusologii) 6:720–725
    [Google Scholar]
  13. Hink W. F. 1970; Established insect cell line from the cabbage looper Trichoplusia ni. Nature, London 226:466–467
    [Google Scholar]
  14. Hirumi H., Hirumj K., McIntosh A. H. 1975; Morphogenesis of a nuclear polyhedrosis virus of the cabbage looper in a continuous cabbage looper cell line. Annals of the New York Academy of Sciences 266:302–326
    [Google Scholar]
  15. Hughes K. M. 1978; The macromolecular lattice of polyhedra. Journal of Invertebrate Pathology 31:217–224
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  17. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  18. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  19. McCarthy W. J., Liu S. H. 1976; Electrophoretic and serological characterization of Porthetria dispar polyhedron protein. Journal of Invertebrate Pathology 28:57–65
    [Google Scholar]
  20. Potter K. N., Faulkner P., MacKinnon E. A. 1976; Strain selection during serial passage of Trichoplusia ni nuclear polyhedrosis virus. Journal of Virology 18:1040–1050
    [Google Scholar]
  21. Skatulla Von U. 1977; Eine neue virose beim Schwammspinner, Lymantria dispar L. (Lep., Lymantriidae). Anz Schadlingskde, Pflanzenhutz, Umwettschutz 50:188–189
    [Google Scholar]
  22. Stairs G. R. 1964; Selection of a strain of insect granulosis virus producing only cubic inclusion bodies. Virology 24:520–521
    [Google Scholar]
  23. Studier F. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  24. Summers M. D. 1975; Biophysical and biochemical properties of baculoviruses. In Baculoviruses for Insect Pest Control: Safety Considerations pp 17–29 Edited by Summers M. D. Engler R., Falcon L. A., Vail P. A. Washington: American Society for Microbiology;
    [Google Scholar]
  25. Vaughn J. L., Goodwin R. H., Tompkins G. J., Mccawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
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