1887

Abstract

Summary

Two morphology mutants designated -5 and -6, of nuclear polyhedrosis virus (NPV) were isolated from virus grown in the presence of -methyl-′-nitro--nitrosoguanidine. Infected cells contained single, large cuboidal inclusion bodies with a crystalline lattice ultrastructure and envelope similar to that of wild-type (wt) polyhedra. The inclusion bodies had low infectivity when fed to larvae. The paracrystalline lattice structure of the mutants was similar to wt, but occluded viruses were rarely found within the mutants. The -5 polyhedrin (mol. wt. 30000) could be distinguished from wt and -6 polyhedrin on the basis of migration in SDS-PAGE gels. Peptide maps of polyhedrin were obtained following digestion with chymotrypsinogen or V8 protease. They were identical for -6 and wt polyhedrin but -5 polyhedrin gave a different pattern. Thus, the altered morphology may be due to a change in polyhedrin composition not detectable in -6 polyhedrin by the methods used here, or it may be the result of a mutation affecting a protein not yet identified.

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/content/journal/jgv/10.1099/0022-1317-50-2-309
1980-10-01
2019-11-14
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-2-309
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