Inborn resistance to orthomyxoviruses due to the allele Mx was investigated at the molecular level in macrophages from A2G mice. In the absence of interferon, Mx-bearing and control cells were equally permissive for M-TUR, a macrophage-adapted strain of influenza A virus. After treatment with various doses of mouse interferon type I, Mx-bearing cells were fully resistant to this virus whereas cells lacking Mx were only marginally protected. Virus attachment and penetration were equally efficient in Mx-carrying and control cells and were not influenced by interferon. However, virus protein synthesis was blocked in Mx-bearing macrophages expressing resistance. These findings indicate that the resistance mechanism specific for influenza viruses realized by Mx in cooperation with interferon inhibits influenza virus replication in macrophages at an early stage following attachment and penetration of the virus.
BoschF. X.,
HayA.,
SkehelJ. J.1978; RNA and protein synthesis in a permissive and an abortive influenza infection. In Negative Strand Viruses and the Host Celt pp 465–474 Edited by
MahyB. W. J.,
BarryR. D.
London and New York: Academic Press;
GalsterR. L.,
LengyelP.1976; Formation and characteristics of reovirus subviral particles in interferon-treated mouse L cells. Nucleic Acids Research 3:581–598
HallerO.,
ArnheiterH.,
GresserI.,
LindenmannJ.1979; Genetically determined, interferon-dependent resistance to influenza virus in mice. Journal of Experimental Medicine 149:601–612
HorisbergerM. A.,
SchulzeC.1974; Properties of an RNA polymerase complex detected in influenza virus-infected cells. Archiv für die gesamte Virusforschung 46:148–155
LindenmannJ.,
DeuelE.,
FanconiS.,
HallerO.1978; Inborn resistance of mice to myxoviruses: macrophages express phenotype in vitro. Journal of Experimental Medicine 147:531–540
RepikP.,
FlamandA.,
BishopD. H. L.1974; Effect of interferon upon primary and secondary transcription of vesicular stomatitis and influenza viruses. Journal of Virology 14:1169–1178
Ter MeulenV.,
LoveR.1967; Virological, immunochemical and cytochemical studies of four HeLa-cell lines infected with two strains of influenza virus. Journal of Virology 1:626–639
ToveyM. G.,
Begon-LoursJ.,
GresserL.1974; A method for the scale production of potent interferon preparations. Proceedings of the Society for Experimental Biology and Medicine 146:809–815