1887

Abstract

SUMMARY

A tissue culture-adapted strain of bovine rotavirus, grown in calf kidney monolayers, has been examined by light and electron microscopy after immunoperoxidase labelling. Some of the characteristic problems associated with pre-embedding methods have been demonstrated. Preparative techniques involving pretreatment of infected cells with a detergent followed by a detergent/fixative combination have enabled virus antigen to be labelled while maintaining satisfactory ultrastructural preservation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-50-1-195
1980-09-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/50/1/JV0500010195.html?itemId=/content/journal/jgv/10.1099/0022-1317-50-1-195&mimeType=html&fmt=ahah

References

  1. Bohn W. 1978; A fixation method for improved antibody penetration in electron microscopical immuno-peroxidase studies. Journal of Histochemistry and Cytochemistry 26:293–297
    [Google Scholar]
  2. Bohn W. 1980; Electron microscopic immunoperoxidase studies on the accumulation of virus antigen in cells infected with Shope fibroma virus. Journal of General Virology 46:439–447
    [Google Scholar]
  3. Chasey D. 1977; Different particle types in tissue culture and intestinal epithelium infected with rotavirus. Journal of General Virology 37:443–451
    [Google Scholar]
  4. Chasey D., Alexander D. J. 1976; Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells. Archives of Virology 52:101–111
    [Google Scholar]
  5. Ciampor F., Bystricka M., Rajcani J. 1974; Ultrastructural localization of influenza virus antigens in infected chick embryo cells as revealed by a peroxidase-labelled antibody method. Archiv für die gesamte Virusforschung 46:341–352
    [Google Scholar]
  6. Dmochowski L., Hoshino M., Shigematsu T., Hiraki S., Priori E. S. 1974 In Viral Immunodiagnosis chapter 12 pp 195–213 Edited by Kurstak E., Morisset R. New York: Academic Press;
    [Google Scholar]
  7. Hall J. G., Birbeck M. S. O., Robertson D., Peppard J., Orlans E. 1978; The use of detergents and immunoperoxidase reagents for the ultrastructural demonstration of internal immunoglobulin in lymph cells. Journal of Immunological Methods 19:351–359
    [Google Scholar]
  8. Kurstak E., Kurstak C. 1974 In Viral Immunodiagnosis chapter i pp 13–21 Edited by Kurstak E., Morisset R. New York: Academic Press;
    [Google Scholar]
  9. Leduc E. H., Wicker R., Avrameas S., Bernhard W. 1969; Ultrastructural localization of SV40T antigen with enzyme-labelled antibody. Journal of General Virology 4:609–614
    [Google Scholar]
  10. Nakane P. K., Kawaoi A. 1974; Peroxidase-labelled antibody. A new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  11. Perre J., Foncin J. F. 1977; An embedding method for monolayer cell cultures for light and electron microscopy. Stain Technology 52:240–242
    [Google Scholar]
  12. Williams M. A. 1977 In Practical Methods in Electron Microscopy vol 6 chapter 3 pp 41–68 Edited by Glauert A. M. Amsterdam: North Holland;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-1-195
Loading
/content/journal/jgv/10.1099/0022-1317-50-1-195
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error