1887

Abstract

SUMMARY

The two dIII fragments of polyoma virus DNA were cloned in the dIII site of plasmid pBR322, and the biological activity of the recombinant plasmids was tested in tissue culture cells. A mixture of recombinant plasmids containing the dIII-A and dIII-B fragments was infectious, but only after cleavage with dIII. Recombinant plasmids containing the dIII-A fragment, but not those containing the dIII-B fragment, induced the transformation of Fischer rat 3T3 cells. These findings indicate that about half of the early region of polyoma virus DNA is not essential for the initiation or the maintenance of transformation.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-50-1-179
1980-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/50/1/JV0500010179.html?itemId=/content/journal/jgv/10.1099/0022-1317-50-1-179&mimeType=html&fmt=ahah

References

  1. Bacheler L. T. 1977; Virus-specific transcription in 3T3 cells transformed by the ts-a mutant of polyoma virus. Journal of Virology 22:54–64
    [Google Scholar]
  2. Bauer W., Vinograd J. 1968; The interaction of closed circular DNA with intercalative dyes. I. The superhelix density of SV40 DNA in the presence and absence of dye. Journal of Molecular Biology 33:141–171
    [Google Scholar]
  3. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W., Crosa J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  4. Chan H. W., Israel M. A., Garon C. F., Rowe W. P., Martin M. A. 1979; Molecular cloning of polyoma virus DNA in Escherichia coli: lambda phage vector system. Science 203:887–892
    [Google Scholar]
  5. Curtiss HI. R., Inoue M., Pereira D., Hsu J. C., Alexander C., Rock L. 1977; Construction and use of safer bacterial host strains for recombinant DNA research. In Molecular Cloning of Recombinant DNA vol 13 pp 99–114 Edited by Scott W., Werner R. Miami Winter Symposia: Academic Press;
    [Google Scholar]
  6. Denhardt D. T. 1966; A membrane-filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  7. Eckhart W. 1977; Complementation between termperature-sensitive (ts) and host range nontransforming (hr-t) mutants of polyoma virus. Virology 77:589–597
    [Google Scholar]
  8. Feunteun J., Sompayrac L., Fluck M., Benjamin T. 1976; Localization of gene functions in polyoma virus DNA. Proceedings of the National Academy of Sciences of the United States of America 73:4169–4173
    [Google Scholar]
  9. Fluck M. M., Staneloni R. J., Benjamin T. L. 1977; Hr-t and ts-a: two early gene functions of polyoma virus. Virology 77:610–624
    [Google Scholar]
  10. Fried M. 1970; Characterization of a temperature-sensitive mutant of polyoma virus. Virology 40:605–617
    [Google Scholar]
  11. Fried M., Griffin B. E., Lund E., Robberson D. L. 1974; Polyoma virus - a study of wild-type, mutant and defective DNAs. Cold Spring Harbor Symposia on Quantitative Biology 39:45–52
    [Google Scholar]
  12. Fried M., Klein B., Murray K., Greenway P., Tooze J., Boll W., Weissman C. 1979; InfeCtivity in mouse fibroblasts of polyoma DNA integrated into plasmid PBR322 or lambdoid phage DNA. Nature, London 279:811–816
    [Google Scholar]
  13. Friedmann T., Laporte P., Esty A. 1978; Nucleotide sequence studies of polyoma DNA. Journal of Biological Chemistry 253:6561–6567
    [Google Scholar]
  14. Griffin B. F., Fried M., Cowie A. 1974; Polyoma DNA: a physical map. Proceedings of the National Academy of Sciences of the United States of America 71:2077–2081
    [Google Scholar]
  15. Grunstein M., Hogness D. S. 1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences of the United States of America 72:3961–3965
    [Google Scholar]
  16. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  17. Hutchinson M. A., Hunter T., Eckhart W. 1978; Characterization of T antigens in polyoma-infected and transformed cells. Cell 15:65–77
    [Google Scholar]
  18. Israel M. A., Chan H. W., Hourihan S. L., Rowe W. P., Martin M. A. 1979a; Biological activity of polyoma viral DNA in mice and hamsters. Journal of Virology 29:990–996
    [Google Scholar]
  19. Israel M. A., Simmons D. T., Hourihan S. L., Rowe W. P., Martin M. A. 1979b; Interrupting the early region of polyoma virus DNA enhances tumorigenicity. Proceedings of the National Academy of Sciences of the United States of America 76:3713–3716
    [Google Scholar]
  20. Israel M. A., Chan H. W., Rowe W. P., Martin M. A. 1979c; Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system. Science 203:883–887
    [Google Scholar]
  21. Ito Y., Brocklehurst J. R., Dulbecco R. 1977; Virus specific proteins in the plasma membrane of cells lytically infected or transformed by polyoma virus. Proceedings of the National Academy of Sciences of the United States of America 74:4666–4670
    [Google Scholar]
  22. Kamen R., Lindstrom D. M., Shure H., Old R. 1974; Virus-specific RNA in cells productively infected or transformed by polyoma virus. Cold Spring Harbor Symposia on Quantitative Biology 39:187–198
    [Google Scholar]
  23. Macpherson L., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  24. Maniatis T., Jeffrey A., Kleid D. G. 1975; Nucleotide sequence of the rightward operator of phage lambda. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
    [Google Scholar]
  25. Miller L., Fried M. 1976; Construction of the genetic map of the polyoma genome. Journal of Virology 18:824–832
    [Google Scholar]
  26. Schaffhausen B. S., Silver J. E., Benjamin T. L. 1978; Tumor antigen(s) in cells productively infected by wild-type polyoma virus and mutant NG-18. Proceedings of the National Academy of Sciences of the United States of America 75:79–83
    [Google Scholar]
  27. Seif R., Cuzin F. 1977; Temperature-sensitive growth regulation in one type of transformed rat cell induced by the ts-a mutant of polyoma virus. Journal of Virology 24:721–728
    [Google Scholar]
  28. Soeda E., Arrand J. R., Smolar N., Griffin B. E. 1979; Sequence from early region of polyoma virus DNA containing viral replication origin and encoding small, middle and (part of) large T antigens. Cell 17:357–370
    [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Stow N. D., Wilkie N. M. 1976; An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. Journal of General Virology 33:447–458
    [Google Scholar]
  31. Thorne H. V., Evans J., Warden D. 1968; Detection of biologically defective molecules in component I of polyoma virus DNA. Nature, London 219:728–730
    [Google Scholar]
  32. Thuring R. W. J., Sanders J. P. M., Borst P. 1975; A freeze-squeeze method for recovering long DNA from agarose gels. Analytical Biochemistry 66:213–220
    [Google Scholar]
  33. Tooze J. (editor) 1973 The Molecular Biology of Tumor Viruses New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-50-1-179
Loading
/content/journal/jgv/10.1099/0022-1317-50-1-179
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error