Macromolecular synthesis in mammalian cells following infection with picorna-viruses has been studied by several groups (Baltimore & Franklin, 1962; Penman et al. 1963; Plagemann, 1968). However, much of the evidence is contradictory and does not lead to a unified hypothesis accounting for the observed effects. This was demonstrated clearly by the work of Plagemann (1968) in which infection of L cells with mengovirus resulted in a rapid inhibition of cellular RNA and protein synthesis, whereas neither process was affected when Novikoff rat hepatoma cells were used. The confusion in this area of biochemistry was reviewed clearly by Martin & Kerr (1968).
Our previous studies (Brown, Martin & Underwood, 1966) indicated that infection of BHK 21 cells with foot-and-mouth disease virus (type SAT 1) inhibited cell protein synthesis within 15 to 30 min. after infection.
BaltimoreD.,
FranklinR. M.1962; The effect of mengovirus infection on the activity of the DNA-dependent RNA polymerase of L-cells. Proc. natn. Acad. Sci., U.S.A. 48:1383
BrownF.,
MartinS. J.,
UnderwoodB.1966; A study of the kinetics of protein and RNA synthesis induced by foot-and-mouth disease virus. Biochim. biophys. Acta 129:166
MartinE. M.,
KerrI. M.1968 Virus-induced changes in host-cell macromolecular synthesis. The Molecular Biology of Viruses p. 15 Ed. by
CrawfordL. V.,
StokerM. G. P.
London: Cambridge University Press;
PenmanS.,
ScherrerK.,
BeckerY.,
DarnellJ. E.1963; Polyribosomes in normal and poliovirus-infected HeLa cells and their relationship to messenger RNA. Proc. natn. Acad. Sci., U.S.A. 49:654
PlagemannP. G. W.1968; Mengovirus replication in Novikoff rat hepatoma and mouse L cells. Effects on synthesis of host cell macromolecules and virus specific synthesis of ribonucleic acid. J. Virol 2:461