1887

Abstract

Phage-associated enzymes which act on capsules of hosts with the production of plaques surrounded by halos have been reported for a number of capsulated Gramnegative bacteria (Bartell, Orr & Lam, 1966; Chakrabarty, Niblack & Gunsalus, 1967). Organisms in the halos are devoid of capsules but are viable and in some instances become sensitive to the action of different phages. This suggests that hidden phage receptors may have been exposed (Sutherland & Wilkinson, 1965). This communication describes the isolation and action of a capsule-depolymerizing enzyme associated with an phage host system.

bacteriophage 6 produces plaques with depressed haloes on the capsulated host strain 6 (Mare, de Klerk & Prozesky, 1966). Haloes are not produced on an uncapsulated spontaneous variant 6 of strain 6. The haloproducing agent is a capsule-destroying enzyme.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-4-551
1969-12-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/4/JV0050040551.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-4-551&mimeType=html&fmt=ahah

References

  1. Adams M. H., Park B. H. 1956; An enzyme produced by a phage-host cell system. II. Properties of the polysaccharide depolymerase. Virology 2:719
    [Google Scholar]
  2. Bartell P. F., Orr T. E., Lam G. K. H. 1966; Polysaccharide depolymerase associated with bacteriophage infection. J. Bact 92:56
    [Google Scholar]
  3. Chakrabarty A. M., Niblack J. F., Gunsalus I. C. 1967; A phage-initiated polysaccharide depolymerase in Pseudomonas putida. Virology 32:532
    [Google Scholar]
  4. Crowle A. J. 1961 Immunodiffusion New York: Academic Press;
    [Google Scholar]
  5. Jann K., Jann B., Ørskov F., Ørskov I., Westphal O. 1965; Immunochemische Untersuchungen an K-antigenen von Escherichia coli. II. Das K-antigen von E. coli 08:K42 (A): H. Biochem Z 342:1
    [Google Scholar]
  6. Maré I. J., de Klerk H. C., Prozesky O. W. 1966; The morphology of Alcaligenes faecalis bacteriophages. J. gen. Microbiol 44:23
    [Google Scholar]
  7. Miller G. L., Blum R., Glennon W. E., Burton A. L. 1960; Measurement of carboxymethyl-cellulase activity. Analyt. Biochem 1:127
    [Google Scholar]
  8. Reissig J. L., Strominger J. L., Leloir L. F. 1955; A modified colorimetric method for the estimation of N-acetyl-amino sugars. J. biol. Chem 217:959
    [Google Scholar]
  9. Sutherland I. W., Wilkinson J. F. 1965; Depolymerases of bacterial exopolysaccharides obtained from phage-infected bacteria. J. gen. Microbiol 39:373
    [Google Scholar]
  10. Tiselius A., Hjertén S., Levin Ö. 1956; Protein chromatography on calcium phosphate columns. Archs Biochem Biophys 65:132
    [Google Scholar]
  11. Wise C. S., Dimler R. J., Davis H. A., Rist C. E. 1955; Determination of easily hydrolyzable fructose units in dextran preparation. Analyt. Chem 27:33
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-4-551
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error