1887

Abstract

SUMMARY

Heat-killed whole cells, purified cell walls, and the three main polymers isolated from cell walls of sp. 607, 14468 and 11758, were examined to find the receptor sites for phage GS-7. Among heat-killed whole cells of insusceptible species, adsorbed the phage at a higher rate than the susceptible sp. 607, whereas did not adsorb the phage at all, suggesting a distinction between phage-resistance (absence of receptor) and phage-immunity (presence of both receptor and repressor).

Each of the three polymers of the cell wall (the lipopolysaccharide of the outer layer, the lipopolysaccharide-lipoprotein complex of the middle layer and the lipopolysaccharide-mucopeptide or mucopeptide complex of the inner layer) contain receptor substances. The lipopolysaccharide moiety isolated from the lipopolysaccharide-lipoprotein complex adsorbed phage at the same rate as the whole complex; implying that the possible receptor substances were the lipopolysaccharide moieties of the three polymers. Delipidation of both cell wall and the lipopolysaccharide-mucopeptide complex resulted in a loss of phage-adsorbing capacity, suggesting that the lipid portion of the lipopolysaccharides may constitute the phage receptors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-4-493
1969-12-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/4/JV0050040493.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-4-493&mimeType=html&fmt=ahah

References

  1. Fischetti V. A., Zabriskie J. B. 1968; Studies on streptococcal bacteriophages. II. Adsorption studies on group A and group C streptococcal bacteriophages. /. exp. Med 127:489
    [Google Scholar]
  2. Goebel W. F., Jesaitis M. A. 1952; The somatic antigen of phage-resistant variants of phase II Shigella sonnei. J. exp. Med 96:425
    [Google Scholar]
  3. Imaeda T., Rieber M. 1969; The relationship between spheroplast formation and lysogeny in mycobacteria. Bact. Proc p. 195
    [Google Scholar]
  4. Imaeda T., Kanetsuna F., Galindo B. 1968; Ultrastructure of cell wall of genus mycobacterium. J. Ultrastruct. Res 25:46
    [Google Scholar]
  5. Jesaitis M. A., Goebel E. F. 1952; The chemical and antiviral properties of the somatic antigen of phase II Shigella sonei. J. exp. Med 96:409
    [Google Scholar]
  6. Jesaitis M. A., Goebel W. F. 1955; Lysis of T4 phage by the specific lipocarbohydrate of phase II Shigella sonnei. J. exp. Med 102:733
    [Google Scholar]
  7. Kanetsuna F. 1968; Chemical analyses of mycobacterial cell wall. Biochim. biophys. Acta 158:130
    [Google Scholar]
  8. Michael J. G. 1968; The surface antigens and phage receptors in Escherichia coli B. Proc. Soc. exp. Biol. Med 128:434
    [Google Scholar]
  9. Oram J. D., Reiter B. 1968; The adsorption of phage to group N Streptococci: the specificity of adsorption and the location of phage receptor substances in cell wall and plasma membrane. J. gen. Virol 3:103
    [Google Scholar]
  10. Rapin A. M. C., Kalckar H. M., Alberico L. 1968; The metabolic basis for masking of receptor-sites on E. coli K-12 for C 21. A lipopolysaccharide core-specific phage. Archs Biochem. Biophys 128:95
    [Google Scholar]
  11. Sellers M. I., Baxter W. L., Runnals H. R. 1962; Growth characteristics of mycobacterio-phages D28 and D29. Can. J. Microbiol 8:389
    [Google Scholar]
  12. Vidaver A. K., Brock T. D. 1966; Purification and properties of bacteriophage receptor material from Streptococcus faecium. Biochim. biophys. Acta 121:298
    [Google Scholar]
  13. Ward D. M., Redmond W. B. 1962; Spotting method of phage typing mycobacteria. Bacterial clearing due to nonspecific action. Am. Rev. resp. Dis 85:883
    [Google Scholar]
  14. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bact 66:688
    [Google Scholar]
  15. Weidel W., Koch G., Bobosch K. 1954; Über die Rezeptorsubstüz für den Phagen T 5. I. ExtraktionundReindarstellung aus E. coli B. Physikalische, Chemische und funktionelle Charak-terisierung. Z. Naturf 9b:573
    [Google Scholar]
  16. Weidel W., Koch G., Lohss F. 1954; Über die Zellmembran von Escherichia coli B. II. Der Rezeptorkomplex fiir die Bakteriophagen T3, T4 und T 7; Vergleichende chemischanalytische Untersuchungen. Z. Naturf 9b:398
    [Google Scholar]
  17. White A., Knight V. 1958; Effect of tween 80 and serum on the interaction of mycobacteriophage D-29 with certain mycobacterial species. Am. Rev. resp. Dis 77:134
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-4-493
Loading
/content/journal/jgv/10.1099/0022-1317-5-4-493
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error