1887

Abstract

SUMMARY

The sequence of changes in human NB and SMH cells that occurred after H-1 virus infection, was studied with the electron microscope. The earliest alterations in NB cells after infection were detected in the nucleolus, after 31 hr. The appeared devoid of its formed elements and was occupied instead with mainly ‘incomplete’ H-1 virus. The was more diffuse than normal and its nucleolar granules more sharply defined. A few ‘incomplete’ and ‘complete’ virus particles were scattered about the nucleus at approximately the same time. The cytoplasm was intact. Shortly thereafter, margination of the nuclear chromatin occurred. The nucleoli became increasingly condensed and shrunken, and apparently formed a doughnut-shaped body with condensed walls containing empty virus. Eventually, most of the nucleolar elements disappeared as the nuclei filled with complete and incomplete virus particles as well as occasional crystals possibly of protein nature. As the nucleolus disappeared, the cytoplasm disintegrated except for a few scattered mitochondria and some recognizable areas of endoplasmic reticulum which contained complete virus particles in linear array. Seventy-seven hr after infection when the nucleus had also broken down, virus was found associated with the disintegrated nucleus, within fragments of endoplasmic reticulum, and attached to thickened and adjoining plasma membranes of the few remaining, apparently uninfected cells.

In SMH cells basically similar changes occurred except that the nucleoli appeared to fragment 31 hr after infection. Later, though most of the nucleolar sections disappeared, a few persisted and formed ‘nucleolar inclusions’ identical to those observed in NB cells. In contrast to the mixture of ‘complete’ and ‘incomplete’ virus observed in NB cells, the virus found in SMH cells was almost exclusively ‘empty’. Virus seen outside the nucleus, either in NB or SMH cells, appeared complete.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-4-485
1969-12-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/4/JV0050040485.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-4-485&mimeType=html&fmt=ahah

References

  1. Almeida J. D., Howatson A. E., Williams M. G. 1962; Electron microscope study of human warts; sites of virus production and nature of the inclusion bodies. J. invest. Derm 38:337
    [Google Scholar]
  2. Bedoya V., Rabson A. S., Grimley P. M. 1968; Growth in vitro of herpes simplex virus in human lymphoma cell lines. J. mtn. Cancer Inst 41:635
    [Google Scholar]
  3. Bernhard W., Granboulan N. 1968 Electron microscopy of the nucleolus in vertebrate cells. The Nucleus vol 3 p. 81 of Ultrastructure of Biological Systems. Ed. by Dalton A. J., Haguenau F. New York: Academic Press;
    [Google Scholar]
  4. Bernhard W., Kasten F.H., Chaney C. 1963; Étude cytochimique et ultrastructurale des cellules infectées par le virus K du rat et le virus H-1. C. r. hebd Séanc. Acad. Sci., Paris 257:1566
    [Google Scholar]
  5. Blailock Z. R., Rabin E. R., Melnick J. L. 1968; Adenovirus myocarditis in mice; an electron microscopie study. Exp. molec. Path 9:84
    [Google Scholar]
  6. Brand K. G., Sylverton J. T. 1962; Results of species-specific hemagglutination tests on ‘transformed’, nontransformed, and primary cell cultures. J. natn. Cancer Inst 28:147
    [Google Scholar]
  7. Crawford L. V., Follett E. A. C., Burdon M. G., Mcgeoch G. J. 1969; The DNA of a minute virus of mice. J. gen. Virol 4:37
    [Google Scholar]
  8. Dunn A. E. G., Oogilvie M. M. 1968; Intranuclear virus particles in human genital wart tissue: observations on the ultrastructure of the epidermal layer. J. Ultrastruct. Res 22:292
    [Google Scholar]
  9. Fawcett D. W. 1966 An Atlas on Fine Structure. The Cell Philadelphia, Pa.: W. B. Saunders Co;
    [Google Scholar]
  10. Flickinger C. J. 1968; The fine structure of the nucleoli of normal and actinomycin D-treated amoeba proteus. J. Ultrastruct. Res 23:260
    [Google Scholar]
  11. Granboulan N., Tournier p., Wixker R., Bernhard W. 1963; An electron microscope study of the development of SV40 virus. J. Cell Biol 17:423
    [Google Scholar]
  12. Greene A. E., Coreeix L. L., Charney J. 1964; A rapid cytotoxic antibody test to determine species of cell cultures. J. natn. Cancer Inst 32:779
    [Google Scholar]
  13. Jacob J. 1968; Involvement of the nucleolus in viral synthesis in the cells of primary renal tumours of Leopard frogs. Cancer. Res 23:2126
    [Google Scholar]
  14. Ledinko N. 1967; Plaque assay of the effects of cytosine arabinoside and 5-iodo-2’-deoxyuridine on the synthesis of H-1 virus particles. Nature, Lond 214:1346
    [Google Scholar]
  15. Ledinko N., Toolan H. W. 1968; Human adenovirus as a ‘helper’ for growth of H-1 virus. J. gen. Virol 2:155
    [Google Scholar]
  16. Ledinko N., Hopkins S., Toolan H. W. 1969; Relationship between potentiation of H-1 growth by human adenovirus 12 and inhibition of the ‘helper’ adenovirus by H-1. J. gen. Virol 5:19
    [Google Scholar]
  17. Mattern C. F. T., Takemoto K. K., Daniel W. A. 1966; Replication of polyoma virus in mouse embryo cells: Electron microscopic observations. Virology 30:242
    [Google Scholar]
  18. Murphy F. A., Harrison A. K., Gray G. W. Jun., Whitfield A. G., Forrester F. T. 1968; St Louis encephalitis virus infection of mice. Lab. Invest 19:652
    [Google Scholar]
  19. Noyes W. E. 1968; Verrucae: virus structure, localization of antigens, and comparison with Shope papilloma. Cancer Res 28:1321
    [Google Scholar]
  20. Portella O. 1963; Hemadsorption and related studies on hamster osteolytic viruses. Arch. ges. Virusforsch 14:277
    [Google Scholar]
  21. Porter K. R., Bonneville M. A. 1968 Fine Structure of Cells and Tissues, 3rd. ed. Philadelphia, Pa.: Lea and Febiger;
    [Google Scholar]
  22. Rabin E. R., Jenson A. B., Melnick J. L. 1968; Herpes simplex virus in mice: electron microscopy of neural spread. Science N.Y. 162:126
    [Google Scholar]
  23. Reddy J., Svoboda D. 1968; The relationship of nucleolar segregation to ribonucleic acid synthesis following the administration of selected hepatocarcinogens. Lab. Invest 19:132
    [Google Scholar]
  24. Robinson D. M., Hetrick F. M. 1969; Single-stranded DNA from the Kilham rat virus. J. gen. Virol 4:269
    [Google Scholar]
  25. Shein H. M., Enders J. F. 1962; Multiplication and cytopathogenicity of simian vacuolating virus 40 in cultures of human tissues. Proc. Soc. exp. Biol. Med 109:495
    [Google Scholar]
  26. Sitori C., Bosisio-Bestetti M. 1967; Nucleolar changes in KB tumour cells infected with herpes simplex. Cancer Res 27:367
    [Google Scholar]
  27. Stenram U., Willen R. 1968; The effect of actinomycin D on RNA synthesis and nucleolar ultrastructure in the liver of rats treated with fluorouracil. Z. Zellforsch. mikrosk. Anat 90:403
    [Google Scholar]
  28. Stone R. S., Shope R. E., Moore D. N. 1959; Electron microscope study of the development of the papilloma virus in the skin of the rabbit. J. exp. Med. no543
    [Google Scholar]
  29. Toolan H. W. 1968; The Picodna Viruses: H, RV and AAV. Int. Rev. exp. Path 6:135
    [Google Scholar]
  30. Toolan H. W., Ledinko N. 1968; Inhibition by H-1 virus of the incidence of tumours produced by adenovirus 12 in hamsters. Virology 35:475
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-4-485
Loading
/content/journal/jgv/10.1099/0022-1317-5-4-485
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error