Vesicular stomatitis virus was grown in a tissue culture line of moth cells and examined by plaque assay in mammalian cells, immunofluorescence and electron microscopy. After infection the virus content of cells increased for 2 to 4 days and then remained relatively constant. There was no obvious cytopathogenic effect on the host cells. Immunofluorescence showed that only a fraction of the cell population was producing a significant amount of virus antigen. This fraction was dependent on the m.o.i. and was equal to ½ when the m.o.i. was 50 p.f.u./cell. At this multiplicity electron microscopy of cell sections revealed the presence of large quantities of virus associated with some of the cells. Budding of vesicular stomatitis virus from the moth cell membrane was also confirmed by electron microscopy.


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