@article{mbs:/content/journal/jgv/10.1099/0022-1317-5-3-451, author = "Neurath, A. R. and Stasny, J. T. and Rubin, B. A. and Hartzell, R. W. and Wiener, F. P.", title = "Gel Filtration on Agarose in the Separation of Adeno-associated Viruses from Adenoviruses and Their Structural Components", journal= "Journal of General Virology", year = "1969", volume = "5", number = "3", pages = "451-454", doi = "https://doi.org/10.1099/0022-1317-5-3-451", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-5-3-451", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Gel filtration on agarose (agar) has been used to separate viruses differing in size (Bengtsson & Philipson, 1964; Čech, 1962; Fridborg et al. 1965; Steere & Ackers, 1962). We therefore tried it for the separation of adeno-associated viruses (AAV) from adenoviruses, including the structural components of the latter. The results presented here indicate that chromatography on Sepharose 2B (Pharmacia, Uppsala, Sweden) represents a useful step for such separation. The source of AAV and adenovirus was similar to that used in previous studies (Hoggan, Blacklow & Rowe, 1966) with adenovirus type 7, strain LLE46+, containing the adenovirus SV40 hybrid and AAV type 1. The viruses were grown in African green monkey kidney cells. The preparation of [3H]thymidine labelled adenovirus type 7 and the measurement of radioactivity are described in a subsequent paper (A. R. Neurath, B. A. Rubin & R. W. Hartzell, 1969, in preparation).", }