@article{mbs:/content/journal/jgv/10.1099/0022-1317-5-3-437, author = "van Rensburg, A. J.", title = "Infection of Spheroplasts With Deoxyriboneucleic Acid From a Proteus Bacteriophage", journal= "Journal of General Virology", year = "1969", volume = "5", number = "3", pages = "437-441", doi = "https://doi.org/10.1099/0022-1317-5-3-437", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-5-3-437", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Infection of protoplasts and spheroplasts by isolated bacteriophage DNA with production of whole phage has been reported for a number of organisms (Riggs & Rosenblum, 1969). This phenomenon has not previously been described in Proteus strains. DNA was prepared from phage 13 vir (Prozesky, de Klerk & Coetzee, 1965) by the technique of Gierer & Schramm (1956). The concentration of DNA, determined by optical extinction at 260 nm. (1 μg. DNA/ml. = 0.0205 absorbency units), ranged from 200 to 600 μg./ml. Sedimentation coefficients of the DNA at concentrations of 10 to 50 μg./ml. were determined in a Spinco model E ultracentrifuge (Rosenblum & Schumaker, 1963). The DNA was homogeneous and an average molecular weight of 26.3 × 106 was calculated. Sedimentation of the DNA through an alkaline sucrose gradient revealed no single-strand breaks.", }