1887

Abstract

Infection of human diploid cells with Simian virus 40 (SV 40) leads to an abortive virus cycle characterized by little virus production, little or no cell destruction, no inhibition of cell replicating capacity, low efficiency of induction of tumour antigen (T-antigen) and eventual transformation of the morphological, chromosomal, and growth characteristics of the cells (Koprowski 1962; Shein & Enders, 1962; Rabson 1962; Weinstein & Moorhead, 1965). In marked contrast, in stationary-phase primary African green monkey kidney (GMK) cells, SV 40 infection leads to high yields of virus, highly efficient induction of T-antigen and extensive cell destruction (Carp & Gilden, 1966). The determination of the cause of these marked differences might clarify the mechanism of the transformation process. Previous results indicated that the virus adsorbed and entered into eclipse phase with equal efficiency for the two cell types (Carp & Gilden, 1966).

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/content/journal/jgv/10.1099/0022-1317-5-3-433
1969-10-01
2022-01-24
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References

  1. Carp R. I. 1967; Thymidine kinase from normal, simian virus 40 transformed and simian virus 40 lytically infected cells. J. Virol 1:912
    [Google Scholar]
  2. Carp R. I., Gilden R. V. 1966; A comparison of the replication cycles of simian virus 40 in human diploid and African green monkey kidney cells. Virology 28:150
    [Google Scholar]
  3. Carp R. I., Sauer G., Sokol F. 1969; The effect of actinomycin D on the transcription and replication of simian virus 40 deoxyribonucleic acid. Virology 37:214
    [Google Scholar]
  4. Gilden R. V., Carp R. I., Taguchi F., Defendi V. 1965; The nature and localization of the sv40-induced complement-fixing antigen. Proc. natn. Acad. Sci. U.S.A. 53:684
    [Google Scholar]
  5. Koprowski H., Pontén J. A., Jensen F., Ravdin R. G., Moorhead P. S., Saksela E. 1962; Transformation of cultures of human tissue infected with simian virus SV 40. J. cell. comp. Physiol 59:281
    [Google Scholar]
  6. McCutchan J. H., Pagano J. S. 1968; Enhancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. J. natn. Cancer Inst 41:351
    [Google Scholar]
  7. Rabson A. S., Malmgren R. A., O’Conor G. T., Kirschstein R. L. 1962; Simian vacuolating virus (SV 40) infection in cell cultures derived from adult human thyroid tissue. J. natn. Cancer Inst 29:1123
    [Google Scholar]
  8. Shein H. M., Enders J. F. 1962; Transformation induced by simian virus 40 in human renal cell cultures. I. Morphology and growth characteristics. Proc. natn. Acad. Sci. U.S.A. 48:1164
    [Google Scholar]
  9. Weinstein D., Moorhead P. S. 1965; Karyology of permanent human cell line, W-18 VA 2, originated by SV 40 transformation. J. cell. comp. Physiol 65:85
    [Google Scholar]
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