1887

Abstract

SUMMARY

When neutral red is added to the liquid medium in tissue cultures, more dye is taken up by healthy cells than by damaged cells. This forms the basis of a method for assessing the relative extent of cell damage in different cultures, the amounts of bound dye being determined directly by inspection of the stained cell sheets, or indirectly by estimating extracted dye colorimetrically. This procedure can be used to measure the effects of a cytocidal virus and can also be applied to the assay of interferons. Here it provides a precise and reproducible method which is unusually sensitive for the assay of mouse interferon, and has also been used to assay rat, chick, rabbit and human interferons.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-3-419
1969-10-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/3/JV0050030419.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-3-419&mimeType=html&fmt=ahah

References

  1. Baron S., Buckler C. E. 1963; Circulating interferon in mice after intravenous injection of virus. Science N.Y. 141:1061
    [Google Scholar]
  2. Baron S., Buckler C. E., Takemoto K. 1966; Rack permitting efficient handling of tissue cultures. Appl. Microbiol 14:1042
    [Google Scholar]
  3. Eagle H. 1959; Amino acid metabolism in mammalian cell cultures. Science N.Y. 130:432
    [Google Scholar]
  4. Finter N. B. 1964a; Interferon production and sensitivity of Semliki Forest virus variants. J. Hyg., Camb 62:337
    [Google Scholar]
  5. Finter N. B. 1964b; A rich source of mouse interferon. Nature, Lond 204:1114
    [Google Scholar]
  6. Finter N. B. 1964c; Quantitative haemadsorption, a new assay technique. Virology 24:589
    [Google Scholar]
  7. Finter N. B. 1966 Interferon assays and standards. In Interferons p. 87 Ed. Finter N. B. Amsterdam: North-Holland Publ. Co;
    [Google Scholar]
  8. Finter N. B. 1969; Methods for screening in vitro and in vivo for agents active against myxo-viruses. Ann. N. Y. Acad. Sci (In the Press.)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-5-3-419
Loading
/content/journal/jgv/10.1099/0022-1317-5-3-419
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error