1887

Abstract

SUMMARY

The BSC/SV 40 virus carrier state was established by infecting monolayers of BSC1 cells with high concentrations of SV40. In these carrier cultures, only 4% of the cells contained intranuclear tumour (T) antigen as determined by the immunofluorescent method, but every cell produced infectious virus. During passage of the BSC/SV40 cultures, a cell line was selected in which all the cells exhibited T antigen and only 0.2−1% of the cells produced infectious virus (transformed state). The BSC1 transformed cells were like the BSC1 parent cells sensitive to challenge with heterologous viruses (attenuated polio 1, herpes simplex and vaccinia), but were resistant to superinfection by the homologous SV40. Cytosine arabinoside, actinomycin D and antiserum to SV40 inhibited virus and T antigens in the BSC/SV40 carrier cultures. In the transformed cells, infectious virus and virus antigen only were inhibited but synthesis of the T antigen remained unaffected. By cell cloning, two virus-free clones were obtained.

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1969-10-01
2022-07-01
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References

  1. Ashkenazi A. 1965; Action of 5-iodo-2-deoxyuridine (IUDR) on in vitro transformation of hamster kidney cells by SV40 virus. Israel J. Med. Sci 1:1012
    [Google Scholar]
  2. Ashkenazi A., Melnick J. L. 1962; Induced latent infection of monkeys with vacuolating SV40 papovavirus. Proc. Soc. exp. Biol. Med III:367
    [Google Scholar]
  3. Black P. H., Rowe W. P. 1963a; Transformation in hamster kidney monolayers by vacuolating virus SV40. Virology 19:107
    [Google Scholar]
  4. Black P. H., Rowe W. P. 1963b; An analysis of SV40 induced transformation of hamster kidney tissue in vitro. I. General characteristics. Proc. natn. Acad. Sci. U.S.A. 50:606
    [Google Scholar]
  5. Black P. H., Rowe W. P. 1963c; SV40 induced proliferation of tissue culture cells of rabbit, mouse and porcine origin. Proc. Soc. exp. Biol. Med 114:721
    [Google Scholar]
  6. Black P. H., Crawford E. M., Crawford L. V. 1964; The purification of Simian virus 40. Virology 24:381
    [Google Scholar]
  7. Black P. H., Rowe W. P., Turner H. C., Huebner R. J. 1963; A specific complement-fixing antigen present in SV40 tumour and transformed cells. Proc. natn. Acad. Sci. U.S.A. 50:1148
    [Google Scholar]
  8. Diderholm H., Berg R., Wesslén T. 1966; Transformation of rat and guinea pig cells in vitro by SV40 and the transplantability of the transformed cells. Int. J. Cancer 1:139
    [Google Scholar]
  9. Diderholm H., Stenvist B., Pontén J., Wesslén T. 1965; Transformation of bovine cells in vitro after inoculation of simian virus 40 or JTS nucleic acid. Expl Cell Res 37:452
    [Google Scholar]
  10. Fernandes M., Moorhead P. S. 1965; Transformation of African green monkey kidney cultures infected with simian vacuolating virus (SV40). Tex. Rep. Biol. Med 23:1965
    [Google Scholar]
  11. Fuks P., Goldblum T., Cymbalista S., Goldblum N. 1967; Physico-chemical properties of SV40 induced tumor (‘T’) antigen. Israel J. Med. Sci 3:912
    [Google Scholar]
  12. Goldblum N., Ravid Z., Becker Y. 1968; Effect of withdrawal of arginine and other amino acids on the synthesis of tumor and viral antigens of SV40 virus. J. gen. Virol 3:143
    [Google Scholar]
  13. Gradwohl R. B. H. 1956 Clinical Laboratory Methods and Diagnosis II, 5th edition. pp. 2046–58 St. Louis: C. B. Mosby;
    [Google Scholar]
  14. Habel K., Jensen F., Pagano J. S., Koprowski H. 1965; Specific complement-fixing tumor antigen in SV40 transformed human cells. Proc. Soc. exp. Biol. Med 118:4
    [Google Scholar]
  15. Hoggan M. D., Rowe W. P., Black P. H., Huebner R. J. 1965; Production of ‘tumor-specific’ antigens by oncogenic viruses during acute cytolytic infections. Proc. natn. Acad. Sci. U.S.A. 53:12
    [Google Scholar]
  16. Hopps H. E., Bernheim B. C., Nisala Z., Tno H. H., Smadel J. E. 1963; Biological characteristics of a continuous kidney cell line derived from the African green monkey. J. Immun 91:416
    [Google Scholar]
  17. Hsiung G. D., Gaylord W. H. Jun. 1961; The vacuolating virus of monkeys. J. exp. Med 114:975
    [Google Scholar]
  18. Jensen F., Koprowski H., Pontén J. A. 1963; Rapid transformation of human fibroblast cultures by simian virus 40. Proc. natn. Acad. Sci. U.S.A. 50:343
    [Google Scholar]
  19. Kit S., Dubbs D. R., Frearson P. M., Melnick J. L. 1966; Enzyme induction in SV40-infected green monkey kidney cultures. Virology 29:69
    [Google Scholar]
  20. Kitahara T., Melnick J. L., Rapp F. 1966; Papovavirus SV40: Similarity of tumor antigens in transformed virus-free hamster cells and in virus-infected monkey cells. Int. J. Cancer 1:249
    [Google Scholar]
  21. Medina D., Sachs L. 1960; The in vitro formation of a stable cell-virus association with polyoma virus. Virology 10:387
    [Google Scholar]
  22. Rabson A. S., Kirschstein R. L., Legallais F. Y. 1965; Autologous implantation of Rhesus monkey cells ‘transformed’ in vitro by simian virus 40. J. natn. Cancer Inst 35:981
    [Google Scholar]
  23. Rapp F., Kitahara T., Butel J. S., Melnick J. L. 1964; Synthesis of SV40 tumor antigen during replication of simian papovavirus (SV40). Proc. natn. Acad. Sci. U.S.A. 52:1138
    [Google Scholar]
  24. Ravid Z., Margalith M., Goldblum N. 1968; Rapid titration of high concentrations of SV40 virus by immunofluorescence. Israel J. Med. Sci 4:945
    [Google Scholar]
  25. Steinbaugh S., Melnick J. L. 1962; Plaque formation by vacuolating virus SV40. Virology 16:348
    [Google Scholar]
  26. Stephalski Z., Knowles B., Koprowski H. 1968; The mechanism of intemuclear transmission of SV40-induced complement fixation antigen in heterokaryocytes. Proc. natn. Acad. Sci. U.S.A. 59:769
    [Google Scholar]
  27. Uchida S., Watanabe S., Kato M. 1966; Incomplete growth of simian virus 40 in African green monkey kidney cultures induced by serial undiluted passages. Virology 38:135
    [Google Scholar]
  28. Uchida S., Yoshuke K., Watanabe S., Furuno A. 1968; Antigen-forming defective viruses of simian virus 40. Virology 34:1
    [Google Scholar]
  29. Vogt M., Dulbecco R. 1960; Virus-cell interaction with a tumor-producing virus. Proc. natn. Acad. Sci. U.S.A. 46:365
    [Google Scholar]
  30. Walker D. L. 1964; The viral carrier state in animal cell cultures. Prog. med. Virol 6:11
    [Google Scholar]
  31. Wallace R. 1967; Viral transformation of monkey kidney cell cultures. Nature, Land 25:768
    [Google Scholar]
  32. Watkins J. F., Dulbecco R. 1967; Production of SV40 virus in heterokaryons of transformed and susceptible cells. Proc. natn. Acad. Sci., U.S.A. 58:1396
    [Google Scholar]
  33. Westphal H., Dulbecco R. 1968; Viral DNA in polyoma and SV40 transformed cell lines. Proc. natn. Acad. Sci. U.S.A. 59:1158
    [Google Scholar]
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