1887

Abstract

Summary

RNA molecules labelled in BHK 21 cells following infection with vesicular stomatitis virus in the presence of Actinomycin D and PO were examined. A complex pattern was obtained when the RNA was centrifuged in sucrose gradients, at least five radioactive peaks being found. These had sedimentation coefficients in sucrose gradients in 0.1 -sodium acetate, pH 5.0, of approx, 38 , 28 , 20 , 12 and less than 4 . Two, 2, 12, 16 and 8% respectively of the radioactive RNA was resistant to ribonuclease 1 g./ml. Treatment of the unfractionated induced RNA with ribonuclease 1 g./ml. yielded a product which had a heterogeneous sedimentation profile (7 to 11 ) in sucrose gradients. Recentrifuging individual fractions of this peak in separate gradients confirmed that the ribonuclease-resistant RNA was heterogeneous. The ribonuclease-resistant RNA also gave a heterogeneous profile in caesium sulphate gradients, with a density range of 1.61 to 1.65 g./ml. The induced RNA which sedimented most slowly in the sucrose gradients could be separated from the labelled cellular 4 RNA by filtration through Sephadex G-75. The base composition of this RNA was significantly different from those of the faster sedimenting induced RNA molecules. Its association with vesicular stomatitis virus infection was confirmed by the failure to detect a similar slowly sedimenting RNA in uninfected cells or in cells infected with foot-and-mouth disease virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-5-2-305
1969-09-01
2024-11-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/5/2/JV0050020305.html?itemId=/content/journal/jgv/10.1099/0022-1317-5-2-305&mimeType=html&fmt=ahah

References

  1. Brown F., Martin S. J. 1965; Base composition of ribosomal ribonucleic acid fractions from five mammalian tissue-culture strains. Biochem. J. 97:20C
    [Google Scholar]
  2. Brown F., Martin S. J., Cartwright B., Crick J. 1967; The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus. J. gen. Virol 1:479
    [Google Scholar]
  3. Cooper P. D., Bellett A. J. D. 1959; A transmissible interfering component of vesicular stomatitis virus preparations. J. gen. Microbiol 21:485
    [Google Scholar]
  4. Crick J., Cartwright B., Brown F. 1969; A study of the interference phenomenon in vesicular stomatitis virus replication. Arch. ges. Virusforsch. (in the Press)
    [Google Scholar]
  5. Huang A. S., Wagner R. R. 1966; Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. J. molec. Biol 22:381
    [Google Scholar]
  6. Martin S. J., Brown F. 1967; Synthesis of ribonucleic acid in baby hamster kidney cells in the presence of actinomycin D. Biochem. J. 105:979
    [Google Scholar]
  7. Szybalski W. 1966; Use of cesium sulfate for equilibrium density gradient centrifugation. In Methods of Enzymology Grossman L., Moldave K. New York: Academic Press;
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-5-2-305
Loading
/content/journal/jgv/10.1099/0022-1317-5-2-305
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error