1887

Abstract

Summary

Rabbit antisera to bovine kidney cells neutralized several naturally occurring strains as well as laboratory modified strains of bovine diarrhoea virus. Antisera to porcine kidney cells failed to neutralize bovine diarrhoea virus even when cultured in porcine kidney cells. These anticellular sera failed to neutralize the viruses of infectious bovine rhinotracheitis, vesicular stomatitis, bovine enterovirus, vaccinia, and parainfluenza-3. Anticellular sera reacted specifically with homologous cells in an indirect immunofluorescent test, but did not form lines of precipitation with cell contents prepared by ultrasonic disruption. These preparations, however, formed multiple lines with antivirus sera, indicating that the antibody produced in rabbits against virus-infected cells differed in some way from the antibody produced in rabbits against whole cells or cell contents.

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1969-09-01
2024-04-20
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References

  1. Amies C. R., Carr J. G. 1939; Immunological experiments with a highly concentrated suspension of Rous I tumor producing agent. J. Path. Bact 49:497
    [Google Scholar]
  2. Axler D. A., Crowell R. L. 1968; Effect of anticellular serum on the attachment of entero- viruses to HeLa cells. J. Virol. 2:813
    [Google Scholar]
  3. Beard D., Beaudreau G. S., Bonar R. A., Sharp D. G., Beard J. W. 1957; Virus of avian erythroblastosis. III. Antigenic constitution and relation to the agent of avian myeloblastosis. J. natn. Cancer Inst 18:231
    [Google Scholar]
  4. Cartwright B., Pearce C. A. 1968; Evidence for a host cell component in vesicular stomatitis virus. J. gen. Virol 2:207
    [Google Scholar]
  5. Cherry W. B., Goldman M., Carski T. R., Moody M. D. 1960; Fluorescent Antibody Techniques in the Diagnosis of Communicable Diseases. U.S. Public Health Service Publication no. 729. U.S. Government Printing Office; Washington D.C.:
    [Google Scholar]
  6. Eckert E. A., Sharp D. G., Beard D., Green I., Beard J. W. 1955; Virus of avian erythro-myeloblastic leukosis. IX. Antigenic constitution and immunologic characterization. J. natn. Cancer Inst 16:593
    [Google Scholar]
  7. Fernelius A. L. 1964; Noncytopathic bovine viral diarrhea viruses detected and titrated by immunofluorescence. Can. J. comp. Med 28:121
    [Google Scholar]
  8. Fernelius A. L. 1966; Heterogeneity of bovine antibodies produced against bovine viral diarrhea (BVD) viruses and against a soluble antigen of BVD produced in cell cultures. J. Immun 96:488
    [Google Scholar]
  9. Fernelius A. L. 1967; Characterization of bovine viral diarrhea viruses by their interactions with susceptible host cells. Ph.D. Thesis, Iowa State University; Ames, Iowa:
    [Google Scholar]
  10. Fernelius A. L. 1968; Characterization of bovine viral diarrhea viruses. II. Ultracentrifugation properties of different strains after various treatments. Arch. ges. Virusforsch 25:219
    [Google Scholar]
  11. Fernelius A. L. 1969; Characterization of bovine viral diarrhea viruses. IV. Sequential development of BVD viral antigen in cell cultures studied by immunofluorescence. Arch. ges. Virusforsch 27:1
    [Google Scholar]
  12. Glazier R. M., Fernelius A. L. 1967; Agar gel staining technique for improved contrast of precipitin lines in immunodiffusion systems. J. biol. photogr. Ass 35:3
    [Google Scholar]
  13. Gutekunst D. E., Malmquist W. A. 1964; Complement-fixing and neutralizing antibody response to bovine viral diarrhea and hog cholera antigens. Can. J. comp. Med 28:19
    [Google Scholar]
  14. Gye W. E., Purdy W. J. 1931; The Cause of Cancer. London: Cassell and Co;
    [Google Scholar]
  15. Habel K., Hornibrook J. W., Gregg N. C., Silverberg R. J., Takemoto K. K. 1958; The effect of anticellular sera on virus multiplication in tissue culture. Virology 5:7
    [Google Scholar]
  16. Kosyakov P. N., Rovnova Z. I., Schavelyova A. P. 1966; Neutralization of myxoviruses by anticellular sera. Acta virol., Prague 10:218
    [Google Scholar]
  17. Laver W. G., Webster R. G. 1966; The structure of influenza viruses. IV. Chemical studies on the host antigen. Virology 30:104
    [Google Scholar]
  18. Malmquist W. A., Fernelius A. L., Gutekunst D. E. 1965; Interference of bovine viral diarrhea virus by hog cholera virus in swine kidney cell cultures. Am. J. vet. Res. 26:1316
    [Google Scholar]
  19. Mengeling W. L., Gutekunst D. E., Fernelius A. L., Pirtle E. C. 1964; Demonstration of an antigenic relationship between hog cholera and bovine viral diarrhea viruses by immunofluo-rescence. Can. J. comp. Med. 27:162
    [Google Scholar]
  20. Ouchterlony O. 1949; Antigen-antibody reactions in gels. Acta path. microbiol. scand. 26:507
    [Google Scholar]
  21. Quersin-Thiry L. 1958; Action of anticellular sera on virus infections. I. Influence on homologous tissue cultures infected with various viruses. J. Immun 81:253
    [Google Scholar]
  22. Rubin H. 1956; An analysis of the apparent neutralization of Rous sarcoma virus with antiserum to normal chick tissues. Virology 2:545
    [Google Scholar]
  23. Van Der Maaten M. J., Packer R. A. 1967; Isolation and characterization of bovine enteric viruses. Am. J. vet. Res. 124:677
    [Google Scholar]
  24. Wildy P., Watson D. H. 1962; Electron microscopic studies on the architecture of animal viruses. Cold Spring Harb. Symp. quant. Biol. 27:25
    [Google Scholar]
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