1887

Abstract

SUMMARY

When a -lysogenic recombination-deficient (rec) mutant of 12, 2463(), was superinfected with ultraviolet-irradiated in the presence of 6-azauracil, there was found among surviving cells a doubly lysogenic form, 2463 ()(), with phenotype. The double lysogen obtained by superinfection of 2463() with cI857 displayed a phenotype at 30° and characteristics at 40°. Superinfection of 2463() with phage 434hy2ch, which lacks the c region of , yielded double lysogens. Since heat inactivation of the repressor in 2463() (cI857 ) produced a form one of the prophages in 2463()() may have been incompletely repressed, hence the expression of its recombination function. For its host?converting effect the superinfecting phage appeared to require the c region of its genome. The results of recombination analysis suggested that in 2463 ()() the first prophage was integrated normally while the second prophage occupied a tandem position more distant than usual, and/or existed in a special relationship conferring on it immunity from or the capacity to overcome the repressor function of the cI product in respect of gene recombination function.

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1969-09-01
2022-08-16
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