1887

Abstract

SUMMARY

Sera from STU mice bearing sarcomas induced by cells producing the Moloney sarcoma-helper-virus (M-MSV/MLV) complex were cytotoxic for these cells as well as for M-MSV non-producer and M-MLV producer cells. Analysis by polyacrylamide gel electrophoresis of I-labelled surface antigens immunoprecipitated with such sera revealed the virus envelope glycoprotein gp71 on the producer cells and an additional antigen of mol. wt 55 K on the M-MSV-transformed producer and non-producer cells. This antigen was not found on non-transformed M-MLV-producing cells and was neither related serologically to structural polypeptides of murine C-type viruses nor to components of embryonal STU mouse fibroblasts and foetal bovine serum.

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1980-08-01
2024-07-23
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References

  1. Aoki T., Stephenson J. R., Aaronson S. A. 1973; Demonstration of a cell-surface antigen associated with murine sarcoma virus by immunoelectron microscopy. Proceedings of the National Academy of Sciences of the United States of America 70:742–746
    [Google Scholar]
  2. Aoki T., Stephenson J. R., Aaronson S. A., HsuA K. C. 1974; Surface antigens of mammalian sarcoma virus-transformed nonproducer cells. Proceedings of the National Academy of Sciences of the United States of America 71:3445–3449
    [Google Scholar]
  3. Bonner W. M., Laskev R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brugge J. S., Erikson R. L. 1977; Identification of a transformation-specific antigen induced by an avian sarcoma virus. Nature, London 269:346–348
    [Google Scholar]
  5. Deleo A. B., Jay G., Appella E., Dubois G. C., Law L. W., Old L. J. 1979; Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proceedings of the National Academy of Sciences of the United States of America 76:2420–2424
    [Google Scholar]
  6. Gahmberg C. G. 1976; External labeling of human erythrocyte glycoproteins. Journal of Biological Chemistry 25:510–516
    [Google Scholar]
  7. Green R. W., Bolognesi D. P., Schafer W., Pister L., Hunsmann G., De Noronha F. 1973; Polypeptides of mammalian oncornaviruses. I. Isolation and serological analysis of polypeptides from murine and feline C-type viruses. Virology 56:565–575
    [Google Scholar]
  8. Hunsmann G., Claviez M., Moennig V., Schwarz H., Schafer W. 1976; Properties of mouse leukemia virus. X. Occurrence of viral structural antigens on the cell surface revealed by a cytotoxicity test. Virology 69:157–168
    [Google Scholar]
  9. Klein G., Klein E., Haughton G. 1966; Variation of antigenic characteristics between different mouse lymphomas induced by the Moloney virus. Journal of the National Cancer Institute 36:607–621
    [Google Scholar]
  10. Lane D. P., Crawford L. V. 1979; T antigen is bound to a host protein in SV40-transformed cells. Nature, London 278:261–263
    [Google Scholar]
  11. Linzer D. L. H., Levine A. J. 1979; Characterization of a 54 K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell 17:43–52
    [Google Scholar]
  12. Melero J. A., Stitt D. Y., Mangel W. F., Carroll R. B. 1979; Identification of new polypeptide species (48–55K) immunoprecipitable by antiserum to purified large T antigen and present in SV40-infected and -transformed cells. Virology 93:466–480
    [Google Scholar]
  13. Philipson L., Anderson P., Olshewsky U., Weinberg R., Baltimore D., Gesteland R. 1978; Translation of MuLV and MSV RNAs in nuclease-treated reticulocyte extracts: enhancement of the gag-pol polypeptide with yeast suppressor tRNA. Cell 13:189–199
    [Google Scholar]
  14. Rohrschneider L. R. 1979; Immunofluorescence of avian sarcoma virus-transformed cells: localization of the src gene product. Cell 16:11–24
    [Google Scholar]
  15. Rubsamen H., Friis R. R., Bauer H. 1979; Src gene products from different strains of avian sarcoma viruses: kinetics and possible mechanisms of heat inactivation of protein kinase activity from cells infected by transformation-defective, temperature-sensitive mutant and wildtype virus. Proceedings of the National Academy of Sciences of the United States of America 76:967–971
    [Google Scholar]
  16. Schafer W., Hunsmann G., Moennig V., De Noronha F., Bolognesi D. P., Green R. W., Huper G. 1975; Polypeptides of mammalian oncornaviruses. II. Characterization of a murine leukemia virus polypeptide (p15) bearing interspecies reactivity. Virology 63:48–59
    [Google Scholar]
  17. Schneider J., Hunsmann G. 1978; Surface expression of murine leukemia virus structural polypeptides on host cells and the virion. International Journal of Cancer 22:204–213
    [Google Scholar]
  18. Schwarz H., Hunsmann G., Moennig V., Schafer W. 1976; Properties of mouse leukemia viruses. XI.Immunoelectron microscopic studies on viral structural antigens on the cell surface. Virology 69:169–176
    [Google Scholar]
  19. Seifert E., Claviez M., Frank H., Hunsmann G., Schwarz H., Schafer W. 1975; Properties of mouse leukemia viruses. XII. Production of substantial amounts of Friend leukemia virus by a suspension tissue culture line (Eveline suspension cells). Zeitschrift für Naturforschung 30c:698–700
    [Google Scholar]
  20. Siegert W., Fenyo E. M., Klein G. 1977; Separation of the Moloney leukemia virus-determined cell surface antigen (MCSA) from known virion proteins associated with the cell membrane. International Journal of Cancer 20:75–82
    [Google Scholar]
  21. Stephenson J. R., Aaronson S. A. 1972; Antigenic properties of murine sarcoma virus-transformed Balb/ 3T3 nonproducer cells. Journal of Experimental Medicine 135:503–515
    [Google Scholar]
  22. Strouk V., Grundner G., Fenyö E. M., Lamon E., Skurzak H., Klein G. 1972; Lack of distinctive surface antigen on cells transformed by murine sarcoma virus. Journal of Experimental Medicine 136:344–352
    [Google Scholar]
  23. Troy F. A., Fenyo E. M., Klein G. 1977; Moloney leukemia virus-induced cell surface antigen: detection and characterization in sodium dodecyl sulfate gels. Proceedings of the National Academy of Sciences of the United States of America 74:5270–5274
    [Google Scholar]
  24. Van zaane D., Dekker-Michielsen M. J. A., Bloemers H. P. J. 1976; Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus: synthesis, identification, and processing. Virology 75:113–129
    [Google Scholar]
  25. Weiland E., Mussgay M. 1976; Detection of cytotoxic lymphoid spleen cells from STU-mice with Moloney sarcoma by a 3H-proline microcytotoxicity assay. Medical Microbiology and Immunology 162:81–87
    [Google Scholar]
  26. Weiland E., Mussgay M., Weiland F. 1978; Nonproducer malignant tumor cells with rescuable sarcoma virus genome isolated from a recurred Moloney sarcoma. Journal of Experimental Medicine 148:408–423
    [Google Scholar]
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