1887

Abstract

SUMMARY

Tissue from symptom-bearing young leaves of plants systemically infected with citrus exocortis viroid (CEV) was investigated with the electron microscope. It was found that, in contrast to a previous report, the appearance of paramural bodies or plasmalemmasomes cannot be considered to be the primary c.p.e. in because these structures are also present at the same frequency in healthy control plants. The plasmalemmasomes in healthy tissue, characterized by vesicular or tubular internal structures, are found at different developmental stages and in the process of spreading between the plasmalemma and cell wall of developing young cells. The viroid-specific cytopathic changes are alterations of the plasmalemmasomes themselves, which may make it difficult to relate them to the corresponding normal structures. The malformed plasmalemmasomes were consistently found in association with the extremely distorted cell walls characteristic of viroid-infected tissue; spreading of plasmalemmasomes was not observed. These findings indicate an intimate functional relationship between plasmalemmasomes and cell wall formation which is disturbed as a consequence of viroid infection.

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1980-08-01
2024-03-28
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References

  1. Brieger E. M., Glauert A. M., Allen J. H. 1959; Cytoplasmic structure in Mycobacterium leprae . Experimental Cell Research 18:418–421
    [Google Scholar]
  2. Cota-robles E. H. 1966; Internal membranes in cells of Escherichia coli. Journal of Ultrastructure Research 16:626–639
    [Google Scholar]
  3. Cronshaw J. 1965; Cytoplasmic fine structure and cell wall development in differentiating xylem elements. In Cellular Ultrastructure of Woody Plants pp 99–124 Edited by Cote W. A. Syracuse: Syracuse University Press;
    [Google Scholar]
  4. Diener T. O. 1971; Potato spindle tuber ‘virus’. IV. A replicating, low molecular weight RNA. Virology 45:411–428
    [Google Scholar]
  5. Diener T. O. 1972; Is the scrapie agent a viroid?. Nature New Biology 235:218
    [Google Scholar]
  6. Diener T. O. 1973; Similarities between the scrapie agent and the agent of potato spindle tuber disease. Annals of Clinical Research 5:268–278
    [Google Scholar]
  7. Edwards M. R. 1962; Plasmalemma and plasmalemmasomes of Listeria monocytogenes. 8. Internal Congress of Microbiology Montreal Abstract 31:
    [Google Scholar]
  8. Esau K., Cheadle V. I., Gill R. H. 1966; Cytology of differentiating tracheary elements. II. Structures associated with cell surfaces. American Journal of Botany 53:765–771
    [Google Scholar]
  9. Fowke L. C., Setterfield G. 1969; Multivesicular structures and cell wall growth. Canadian Journal of Botany 47:1873–1877
    [Google Scholar]
  10. Gibbs C. J. Jun, Gajdusek D. C., Letarjet R. 1978; Unusual resistance to ionizing radiation of the viruses of kuru, Creutzfeldt-Jakob disease, and scrapie. Proceedings of the National Academy of Sciences of the United States of America 75:6268–6270
    [Google Scholar]
  11. Giesbrecht P. 1960; Ober organisierte Mitochondrien und andere Feinstrukturen von Bacillus megaterium . Zentralblatt fur Bakteriologie, Parasitenkunde Infektionskrankheiten und Hygiene, Abteilung I Originate A 179:538–582
    [Google Scholar]
  12. Gross H. J., Domdey H., Lossow C., Jank P., Raba M., Alberty H., Sänger H. L. 1978; Nucleotide sequence and secondary structure of potato spindle tuber viroid. Nature, London 273:203–208
    [Google Scholar]
  13. Halperin W., Jensen W. A. 1967; Ultrastructural changes during growth and embryogenesis in carrot cell cultures. Journal of Ultrastructure Research 18:428–443
    [Google Scholar]
  14. Jensen W. A. 1965; The composition and ultrastructure of the nucellus in cotton. Journal of Ultrastructure Research 13:112–128
    [Google Scholar]
  15. Langowski J., Henco K., Riesner D., Sänger H. L. 1978; Common structural features of different viroids: serial arrangement of double helical sections and internal loops. Nucleic Acids Research 5:1589–1610
    [Google Scholar]
  16. Luft J. H. 1961; Improvements in epoxy resin embedding methods. Journal of Biophysical and Biochemical Cytology 9:409–414
    [Google Scholar]
  17. Mahlberg P., Olson K., Walkinshaw C. 1971; Origin and development of plasma membrane derived invaginations in Vinca rosea L . American Journal of Botany 58:407–416
    [Google Scholar]
  18. Malone T. G., Marsh R. F., Hanson R. P., Semancik J. S. 1979; Evidence for the low molecular weight nature of scrapie agent. Nature, London 278:575–576
    [Google Scholar]
  19. Marchant R., Robards A. W. 1968; Membrane systems associated with the plasmalemma of plant cells. Annals of Botany 32:457–471
    [Google Scholar]
  20. Marsh R. F., Malone T. G., Semancik J. S., Lancaster W. D., Hanson R. P. 1978; Evidence for an essential DNA component in the scrapie agent. Nature, London 275:146–147
    [Google Scholar]
  21. Millonig G. 1962; Further observations on a phosphate buffer for osmium solutions in fixation. V. International Congress of Electron Microscopy; New York: p 8
    [Google Scholar]
  22. Mühlbach H.-P., Sänger H. L. 1977; Multiplication of cucumber pale fruit viroid in inoculated tomato leaf protoplasts. Journal of General Virology 35:377–386
    [Google Scholar]
  23. Mühlbach H. P., Sänger H.-L. 1979; Viroid replication is inhibited by a-amanitin . Nature, London: 278185–188
    [Google Scholar]
  24. Peat A., Banbury G. H. 1967; Ultrastructure protoplasmatic streaming growth and tropisms of Phyco-myces sporangiophores. New Phytologist 66:475–484
    [Google Scholar]
  25. Prusiner S. B., Hadlow W. J., Eklund C. M., Race R. E. 1977; Sedimentation properties of the scrapie agent. Proceeding of the National Academy of Sciences of the United States of America 74:4656–4660
    [Google Scholar]
  26. Prusiner S. B., Hadlow W. J., Eklund C. M., Race R. E., Cochran P. S. 1978a; Sedimentation characteristics of the scrapie agent from murine spleen and brain. Biochemistry 17:4987–4992
    [Google Scholar]
  27. Prusiner S. B., Hadlow W. J., Garfin D. E., Cochran P. S., Barlnger J. R., Race R. E., Eklund C. M. 1978b; Partial purification and evidence for multiple molecular forms of the scrapie agent. Biochemistry 17:4993–4999
    [Google Scholar]
  28. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  29. Riesner D., Henco K., Rokohl U., Klotz G., Kleinschmidt A. K., Domdey H., Jank P., Gross H. I., Sänger H. L. 1979; Structure and structure formation of viroids. Journal of Molecular Biology 133:85–115
    [Google Scholar]
  30. Robards A. W. 1968; On the ultrastructure of differentiating secondary xylem in willow. Protoplasma 65:449–464
    [Google Scholar]
  31. Sänger H. L. 1972; An infection and replicating RNA of low molecular weight: the agent of the exocortis disease of citrus. Advances in the Biosciences 8:103–116
    [Google Scholar]
  32. Sänger H. L., Klotz G., Riesner D., Gross H. J., Kleinschmidt A. K. 1976; Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proceeding of the National Academy of Sciences of the United States of America 78:3852–3856
    [Google Scholar]
  33. Semancik J. S., van der wouDe W. J. 1976; Exocortis viroid: cytopathic effects at the plasma membrane in association with pathogenic RNA. Virology 69:719–726
    [Google Scholar]
  34. Semancik J. S., Tsuruda D., Zaner L., Geelen J. L. M. C., Weathers J. G. 1976; Exocortis disease: Subcellular distribution of pathogenic (viroid) RNA. Virology 69:669–676
    [Google Scholar]
  35. Takahashi T., Diener T. O. 1975; Potato spindle tuber viroid. XIV. Replication in nuclei isolated from infected leaves. Virology 64:106–114
    [Google Scholar]
  36. Tu J. C., Hiruki C. 1971; Electron microscopy of cell wall thickening in local lesions of potato virus in infected red kidney bean. Phytopathology 61:862–868
    [Google Scholar]
  37. Walker W. S., Bisalputra T. 1967; The fine structure of vesicles associated with the cell surface in Helianthus shoot tissue. Canadian Journal of Botany 45:2103–2108
    [Google Scholar]
  38. Weathers L. G., Greer F. C., Harjung M. K. 1967; Transmission of exocortis virus of citrus to herbaceous hosts. Plant Disease Reporter 51:868–871
    [Google Scholar]
  39. Wheeler H. 1974; Cell wall and plasmalemma modification in diseased and injured plant tissue. Canadian Journal of Botany 52:1005–1009
    [Google Scholar]
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