1887

Abstract

SUMMARY

Rabbit cornea cells (RC-60) restrict the reproduction of vesicular stomatitis virus (VSV) (Thacore & Youngner, 1975). In cells infected with VSV alone, an inhibition in the synthesis of VSV genome RNA is observed. A number of parameters which could affect virus RNA synthesis have been examined: virus transcription and post-transcriptional modification, translation, modification of proteins and migration of the G protein to the surface of the cell; they all appear to be normal, although somewhat diminished, in the restricted system. In these cells, therefore, it is the replication of VSV RNA that is directly inhibited, although limited synthesis of both (+) and (-) strand genome length RNA does occur. When the cells are co-infected with rabbit poxvirus (RPV) as a helper virus, however, VSV production is normal. Our studies suggest that RPV plays a role in the maturation as well as in the replication of VSV RNA in RC-60 cells. Certain mutants of RPV have been found to lack the helper function and are unable to convert the RC-60 cells into a permissive host for VSV. These mutants should facilitate the elucidation of the mechanism by which RPV is able to overcome the restriction in these cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-49-2-273
1980-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/49/2/JV0490020273.html?itemId=/content/journal/jgv/10.1099/0022-1317-49-2-273&mimeType=html&fmt=ahah

References

  1. Banerjee A. K., Moyer S. A., Rhodes D. P. 1974; Studies on the in vitro adenylation of RNA by vesicular stomatitis virus. Virology 61:547–558
    [Google Scholar]
  2. Bonner W. M., Laskey R. A. 1974; A film detection method for 3H labeled proteins and nucleic acid in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  3. Chen C., Crouch N. A. 1978; Shope fibroma virus facilitation of VSV adsorption and replication in non-permissive cells. Virology 85:43–62
    [Google Scholar]
  4. Clinton G. M., Burge B. W., Huang A. S. 1978; Effects of phosphorylation and pH on the association of NS protein with VSV cores. Journal of Virology 27:340–346
    [Google Scholar]
  5. Clinton G. M., Burge B. W., Huang A. S. 1979; Phosphoproteins of vesicular stomatitis virus: identity and interconversion of phosphorylated forms. Virology 99:84–94
    [Google Scholar]
  6. Crouch N. A. 1978; Replication of VSV facilitated in non-permissive cells by early functions of shope fibroma virus (40026). Proceedings of the Society for Experimental Biology and Medicine 157:225–230
    [Google Scholar]
  7. Fenner F., Sambrook J. F. 1966; Conditional lethal mutants of RPV. II. Mutants (p) that fail to multiply in PK-2a cells. Virology 28:600–609
    [Google Scholar]
  8. Gibson R., Leavitt R., Kornfeld S., Schlesinger S. 1978; Synthesis and infectivity of VSV containing nonglycosylated G protein. Cell 13:671–679
    [Google Scholar]
  9. Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B. A., Kohne D., Lazzarini R., Scolnick E. 1976; Long term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. Journal of General Virology 33:193–211
    [Google Scholar]
  10. Kessler S. W. 1975; Rapid isolation of antigens from cells with a staphylococcal protein A-antibody absorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage cf structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Leavitt R., Schlesinger S., Kornfeld S. 1977; Impaired intracellular migration and altered solubility of non-phosphorylated glycoproteins of VSV and Sindbis virus. Journal of Biological Chemistry 252:9018–9023
    [Google Scholar]
  13. Marcus P. I., Engelhardt D. L., Hunt J. M., Sekeluck M. J. 1971; Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science 174:593–598
    [Google Scholar]
  14. Moyer R. W., Rothe C. T. 1980; The white pock mutants of rabbit poxvirus. I. Spontaneous host range mutants contain deletions. Virology 102:119–132
    [Google Scholar]
  15. Moyer S. A., Banerjee A. K. 1975; Messenger RNA species synthesized in vitro by the virion associated polymerase of vesicular stomatitis virus. Cell 4:37–43
    [Google Scholar]
  16. Moyer S. A., Banerjee A. K. 1976; In vivo methylation of vesicular stomatitis virus and host-cell messenger RNA species. Virology 70:339–351
    [Google Scholar]
  17. Moyer S. A., Grubman M. J., Ehrenfeld E., Banerjee A. K. 1975; Studies on the in vivo and in vitro messenger RNA species of vesicular stomatitis virus. Virology 67:463–473
    [Google Scholar]
  18. Nowakowski M., Bloom B. R., Ehrenfeld E., Summers D. F. 1973; Restricted replication of VSV in human lymphoblastoid cells. Journal of Virology 12:1272–1278
    [Google Scholar]
  19. Obijeski J. F., Marchenko A. T., Bishop D. H. L., Cann B. W., Morphy F. A. 1974; Comparative electrophoretic analysis of virus proteins of four rhabdoviruses. Journal of General Virology 22:21–33
    [Google Scholar]
  20. Rose J. K. 1975; Heterogenous 5′ terminal structures occur on vesicular stomatitis virus mRNAs. Journal of Biological Chemistry 250:8098–8104
    [Google Scholar]
  21. Rose J. K., Knipe D. 1975; Nucleotide sequence complexities, molecular weights and poly(A) content of the vesicular stomatitis virus mRNA species. Journal of Virology 15:994–1003
    [Google Scholar]
  22. Simonsen C. C., Batt-Humphries S., Summers D. F. 1979a; RNA synthesis of vesicular stomatitis virus infected cells: in vivo regulation of replication. Journal of Virology 31:124–132
    [Google Scholar]
  23. Simonsen C. C., Hill V. M., Summers D. F. 1979b; Further characterization of the replicative complex of vesicular stomatitis virus. Journal of Virology 31:494–505
    [Google Scholar]
  24. Soria M., Little S. P., Huang A. S. 1974; Characterization of vesicular stomatitis virus nucleocapsids. I. Complementary 40S RNA molecules in nucleocapsids. Virology 61:270–280
    [Google Scholar]
  25. Thacore H. R., Youngner J. S. 1973a; Rescue of VSV from interferon induced resistance by super-infection with vaccinia virus. I. Rescue in cell cultures from different species. Virology 56:505–511
    [Google Scholar]
  26. Thacore H. R., Youngner J. S. 1973b; Rescue of VSV from interferon induced resistance by super-infection with vaccinia virus. II. Effect of UV-inactivated vaccinia and metabolic inhibitors. Virology 56:512–522
    [Google Scholar]
  27. Thacore H. R., Youngner J. S. 1975; Abortive infection of a rabbit cornea cell line by VSV: conversion to productive infection by superinfection by vaccinia virus. Journal of Virology 16:322–329
    [Google Scholar]
  28. Wagner R. R. 1975; Reproduction of rhabdoviruses. In Comprehensive Virology vol 4 pp 1–93 Edited by Fraenkel-Conrat H., Wagner R. New York: Plenum;
    [Google Scholar]
  29. Wertz G. W., Levine M. 1973; RNA synthesis by vesicular stomatitis virus and a small plaque mutant: effects of cycloheximide. Journal of Virology 12:253–264
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-49-2-273
Loading
/content/journal/jgv/10.1099/0022-1317-49-2-273
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error