@article{mbs:/content/journal/jgv/10.1099/0022-1317-49-1-97, author = "van Berlo, M. F. and Horzinek, M. C. and van der Zeijst, B. A. M.", title = "Temperature-sensitive Mutants of Equine Arteritis Virus", journal= "Journal of General Virology", year = "1980", volume = "49", number = "1", pages = "97-104", doi = "https://doi.org/10.1099/0022-1317-49-1-97", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-49-1-97", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Seventeen temperature-sensitive mutants of equine arteritis virus, a nonarthropod-borne togavirus, have been isolated. 5-Fluorouracil, 0-methylhydroxylamine and ethyl methanesulphonate were used as mutagens. The mutants were characterized by their ability to synthesize virus RNA and virus proteins at the permissive (35 °C) and restrictive temperature (40 °C) using autoradiography of cells labelled with 3H-uridine in the presence of actinomycin D and immunofluorescence respectively. Among the mutants, four were unable to synthesize virus RNA and virus proteins at 40 °C (RNA−/protein−). The other mutants were RNA−/protein+ (3); RNA±/protein− (2); RNA+/protein+ (6) and RNA+/protein− (1).", }