1887

Abstract

SUMMARY

Seventeen temperature-sensitive mutants of equine arteritis virus, a nonarthropod-borne togavirus, have been isolated. 5-Fluorouracil, 0-methylhydroxylamine and ethyl methanesulphonate were used as mutagens. The mutants were characterized by their ability to synthesize virus RNA and virus proteins at the permissive (35 °C) and restrictive temperature (40 °C) using autoradiography of cells labelled with H-uridine in the presence of actinomycin D and immunofluorescence respectively. Among the mutants, four were unable to synthesize virus RNA and virus proteins at 40 °C (RNA/protein). The other mutants were RNA/protein (3); RNA/protein (2); RNA/protein (6) and RNA/protein (1).

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/content/journal/jgv/10.1099/0022-1317-49-1-97
1980-07-01
2022-01-27
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