1887

Abstract

SUMMARY

Conditions are described for the production of 0.3 to 0.7 NIH mouse reference standard units of interferon per cell from Ehrlich ascites tumour cells cultured as monolayers and induced by infection with Newcastle disease virus (NDV). Inclusion of theophylline (6 m) in the medium increased the interferon yield three to four times. Cells infected with NDV started to lyse at about 15 h p.i., but infected, theophylline-treated cells lysed only 24 h p.i.

Several other methylxanthines (e.g. theobromine, caffeine and isobutylmethylxanthine), when tested at concentrations similar to that of theophylline, did not boost interferon production. Dibutyryl cyclic AMP (10 to 10 ) did not substitute for theophylline in increasing interferon production, and, if used together with theophylline, did not cause further enhancement.

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1980-07-01
2024-12-09
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References

  1. Dianzani F., Neri P., Zucca M. F. 1972; Effect of dibutyryl cyclic AMP on interferon production by cells treated with viral or nonviral inducers. Proceedings of the Society for Experimental Biology and Medicine 140:1375–1378
    [Google Scholar]
  2. Finter N. B. 1973 In Interferons and Interferon Inducers Amsterdam: North Holland;
    [Google Scholar]
  3. Gupta S. L., Sopori M. L., Lengyel P. 1973; Inhibition of protein synthesis directed by added viral and cellular messenger RNAs in extracts of interferon-treated Ehrlich ascites tumor cells. Location and dominance of the inhibitor(s). Biochemical and Biophysical Research Communications 54:777–783
    [Google Scholar]
  4. Kawakita M., Cabrer B., Taira H., Rebello M., Slattery E., Weideli H., Lengyel P. 1978; Purification of interferon from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 253:598–602
    [Google Scholar]
  5. Lauzon G. J., Kulshrestha S., Starr L., Bar H.-P. 1976; On the accumulation of adenosine 3′:5′-monophosphate in Ehrlich cells and adenylate cyclase desensitization in response to epinephrine. Journal of Cyclic Nucleotide Research 2:99–114
    [Google Scholar]
  6. Mecs L. 1973; Regulation of interferon production in vitro and in vivo by influencing the adenylate cyclase system. Acta Microbiologica Academiae Scientiarum Hungaricae 20:42
    [Google Scholar]
  7. Reizin F. N., Roikhel V. M., Chumakov M. P. 1975; The influence of substances changing the intracellular concentration of cyclic adenosine 3′5′-monophosphate on interferon synthesis in chick embryo cell culture. Archives of Virology 49:307–315
    [Google Scholar]
  8. Robison G. A., Butcher R. W., Sutherland E. W. 1968; Cyclic AMP. Annual Review of Biochemistry 37:149–174
    [Google Scholar]
  9. Roman J. M., Simon E. H. 1976; Defective interfering particles in monolayer-propagated Newcastle disease virus. Virology 69:29–303
    [Google Scholar]
  10. Sehgal E., Tamm I., Vilcek J. 1975; Human interferon production: superinduction by 5, 6-dichloro-I-β-d-ribofuranosylbenzimidazole. Science 190:282–284
    [Google Scholar]
  11. Smith P. A., Weight F. F., Levine R. A. 1979; Potentiation of Ca2+-dependent K+ activation by theophylline is independent of cyclic nucleotide elevation. Nature, London 280:400–402
    [Google Scholar]
  12. Spandidos D. A., Graham A. F. 1976; Physical and chemical characterization of an avian reovirus. Journal of Virology 19:968–976
    [Google Scholar]
  13. Stampfer M., Baltimore D., Huang A. S. 1971; Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus. Journal of Virology 7:409–411
    [Google Scholar]
  14. Stewart W. E. II 1979 In The Interferon system Wien: Springer-Verlag;
    [Google Scholar]
  15. Tan Y. H., Armstrong J. A., Ke Y. M., Ho M. 1970; Regulation of cellular interferon production: enhancement by antimetabolites. Proceedings of the National Academy of Sciences of the United States of America 67:464–471
    [Google Scholar]
  16. Tan Y. H., Armstrong J. A., Ho M. 1971; Accentuation of interferon production by metabolic inhibitors and its dependence on protein synthesis. Virology 44:503–509
    [Google Scholar]
  17. Van Der Heide L., Geissler J., Bryant E. S. 1974; Infectious tenosynovitis: serologic and histopathologic response after experimental infection with a Connecticut isolate. Avian Diseases 18:289–296
    [Google Scholar]
  18. Van Venrooij W. J. W., Henshaw E. C., Hirsch C. A. 1970; Nutritional effects on the polyribosome distribution and rate of protein synthesis in Ehrlich ascites tumor cells in culture. Journal of Biological Chemistry 245:5947–5953
    [Google Scholar]
  19. Vassef A., Beaud G., Paucker K., Lengyel P. 1973; Interferon assay based on the inhibition of doublestranded reovirus RNA accumulation in mouse L cells. Journal of General Virology 19:81–87
    [Google Scholar]
  20. Vilcek J., Rossman T. G., Varacalli F. 1969; Differential effects of actinomycin D and puromycin on the release of interferon induced by double-stranded RNA. Nature, London 222:682–684
    [Google Scholar]
  21. Wiranowska-stewart M., Chudzio T., Stewart W. E. II 1977; Repeated ‘superinduction’ of interferon in human diploid fibroblast cultures. Journal of General Virology 37:221–223
    [Google Scholar]
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