RT Journal Article SR Electronic(1) A1 Kraaijeveld, Cornelis A. A1 Madge, M. Hilary A1 Macnaughton, Malcolm R.YR 1980 T1 Enzyme-linked Immunosorbent Assay for Coronaviruses HCV 229E and MHV 3 JF Journal of General Virology, VO 49 IS 1 SP 83 OP 89 DO https://doi.org/10.1099/0022-1317-49-1-83 PB Microbiology Society, SN 1465-2099, AB SUMMARY The antigenic relationship between human coronavirus strain 229E (HCV 229E) and mouse hepatitis virus strain 3 (MHV 3) was studied by means of the indirect form of enzyme-linked immunosorbent assay (ELISA). A cross-reaction was found with hyperimmune rabbit sera between HCV 229E and MHV 3 which may be due to the adherence of bovine serum components from tissue culture media, which were present on virus particles even after extensive purification. No cross-reaction was observed with immune sera absorbed with bovine serum, or with HCV 229E grown in tissue culture without serum. This indirect ELISA with HCV 229E may prove to be useful for studies with human sera., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-49-1-83