1887

Abstract

Summary

The genomes of phages 5006M, kanamycin resistance transducing variant 5006 M HFT and kanamycin-ampicillin resistance transducing variant 5006M HFT have been compared. Homo- and heteroduplex and partial denaturation mapping analyses were performed. The results confirm a sequential headful packaging mechanism, facilitate mapping of the ampicillin resistance marker, demonstrate a hairpin loop structure in both variants, reveal a common insertion site for 8 to 9 × 10 mol. wt. non-phage DNA in both variants and implicate a role for the non-inducible cryptic host strain prophage 5006M in the generation cycle of the variant phages.

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/content/journal/jgv/10.1099/0022-1317-49-1-33
1980-07-01
2019-10-19
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-49-1-33
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