1887

Abstract

SUMMARY

In contrast to biologically active DI particles, neither u.v.-inactivated standard virus nor either of two different homologous u.v.-inactivated DI particles showed any prophylactic effect when injected intracerebrally into mice concomitantly challenged with VSV. Although u.v.-inactivated DI particles did not prevent death when given with the challenge virus, they did significantly lengthen the time until death occurred. Also, both u.v.-inactivated standard virus and DI particles protected mice against late challenge (at 3 or 10 days after treatment). Dosage titrations of preparations of two different active DI particles showed significant prophylaxis against simultaneous challenge with numbers of DI particles 10- to 100-fold lower than those which gave no prophylaxis when u.v.-inactivated. Thus, prophylaxis in this system required biologically active DI particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-49-1-215
1980-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/49/1/JV0490010215.html?itemId=/content/journal/jgv/10.1099/0022-1317-49-1-215&mimeType=html&fmt=ahah

References

  1. Crick J., Brown F. 1977; In vivo interference in vesicular stomatitis virus infection. Infection and Immunity 15:354–359
    [Google Scholar]
  2. Crick J., Brown F. 1978; Interference and persistence in mice injected with vesicular stomatitis virus. In Negative Strand Viruses and the Host Cell pp 583–589 Edited by Mahy B. W. H., Barry R. D. London: Academic Press;
    [Google Scholar]
  3. Dimmock N. J., Kennedy S. I. T. 1978; Prevention of death in Semliki Forest virus-infected mice by administration of defective-interfering Semliki Forest virus. Journal of General Virology 39:231–242
    [Google Scholar]
  4. Doyle M., Holland J. J. 1973; Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 70:2105–2108
    [Google Scholar]
  5. Faulkner G., Dubois-Dalcq M., Hoogke-Peters E., Mcfarland H. F., Lazzarini R. A. 1979; Defective interfering particles modulate VSV infection of dissociated neuron cultures. Cell 17:979–991
    [Google Scholar]
  6. Frey T. K., Jones E. V., Cardamone J. J. Jun, Youngner J. S. 1979; Induction of interferon in L cells by defective interfering (DI) particles of vesicular stomatitis virus: lack of correlation with content of (±) snapback RNA. Virology 99:95–102
    [Google Scholar]
  7. Holland J. J., Villarreal L. P. 1975; Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology 67:438–449
    [Google Scholar]
  8. Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B. A., Kohne D., Lazzarini R., Scolnick E. 1976; Long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. Journal of General Virology 33:193–211
    [Google Scholar]
  9. Huang A. S., Baltimore D. 1970; Defective viral particles and viral disease processes. Nature, London 226:325–327
    [Google Scholar]
  10. Marcus P. I., Sekellick M. J. 1977; Defective interfering particles with covalently linked (±) RNA induced interferon. Nature, London 266:815–819
    [Google Scholar]
  11. Mims C. A. 1956; Rift Valley fever in mice. IV. Incomplete virus; its production and properties. British Journal of Experimental Pathology 37:129–143
    [Google Scholar]
  12. Perrault J., Leavitt R. W. 1977; Characterization of snap-back RNAs in vesicular stomatitis defective interfering virus particles. Journal of General Virology 38:21–34
    [Google Scholar]
  13. Popescu M., Lehmann-Grube F. 1977; Defective interfering particles in mice infected with lymphocytic choriomeningitis virus. Virology 77:78–83
    [Google Scholar]
  14. Spandidos D. A., Graham A. F. 1976; Generation of defective virus after infection of newborn rats with reovirus. Journal of Virology 20:234–247
    [Google Scholar]
  15. Von Magnus P. 1951; Propagation of the PR 8 strain of influenza virus in chick embryos. 3. Properties of the incomplete virus produced in serial passages of undiluted virus. Acta Pathologica et Microbiologica Scandinavica 29:157–181
    [Google Scholar]
  16. Von Magnus P. 1954; Incomplete forms of influenza virus. Advances in Virus Research 2:59–78
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-49-1-215
Loading
/content/journal/jgv/10.1099/0022-1317-49-1-215
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error