Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenotopes: (i) transformed MSV/MLV producers; (ii) non-transformed MLV producers; (iii) transformed non-producers. We have also selected non-transformed revertants from one of the non-producer clones. This series of lines allows the study of the expression of the virus genome against a constant background of cellular gene expression. We have further characterized the lines with regard to anchorage dependence of growth, tumorigenicity and the presence of a rescuable sarcoma genome. The non-producer clones are uniform in their transformed properties. The revertants contain rescuable sarcoma virus, biologically indistinguishable from the original transforming virus, implying that the reversion is due to a change in cellular rather than virus genetic information.
AveryR. J.,
LevyJ. A.1978; Relationship of endogenous murine xenotropic type C virus production to spontaneous transformation of cultured cells. Journal of General Virology 39:427–435
GreenbergerJ. S.,
AaronsonS. A.1974; Morphologic revertants of murine sarcoma virus transformed non-producer Balb/3T3: selective techniques for isolation and biological properties in vitro and in vivo. Virology 57:339–346
HartleyJ. W.,
RoweW. P.1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 55:780–787
PuckT. T.,
KaoF. T.1967; Genetics of somatic mammalian cells. V. Treatment with BudR and visible light for isolation of nutritionally deficient mutants. Proceedings of the National Academy of Sciences of the United States of America 58:1227–1234
StephensonJ. R.,
AaronsonS. A.1972; Antigenic properties of murine sarcoma virus transformed Balb/ 3T3 non-producer cells. Journal of Experimental Medicine 139:503–575