1887

Abstract

SUMMARY

Starting with cloned NIH 3T3 mouse cells we have isolated a series of related lines infected with the Kirsten murine sarcoma/leukaemia (MSV/MLV) virus complex. These lines exhibit all three possible infected cell phenotopes: (i) transformed MSV/MLV producers; (ii) non-transformed MLV producers; (iii) transformed non-producers. We have also selected non-transformed revertants from one of the non-producer clones. This series of lines allows the study of the expression of the virus genome against a constant background of cellular gene expression. We have further characterized the lines with regard to anchorage dependence of growth, tumorigenicity and the presence of a rescuable sarcoma genome. The non-producer clones are uniform in their transformed properties. The revertants contain rescuable sarcoma virus, biologically indistinguishable from the original transforming virus, implying that the reversion is due to a change in cellular rather than virus genetic information.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-49-1-105
1980-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/49/1/JV0490010105.html?itemId=/content/journal/jgv/10.1099/0022-1317-49-1-105&mimeType=html&fmt=ahah

References

  1. Aaronson S. A., Rowe W. P. 1970; Non-producer clones of murine sarcoma virus transformed Balb/3T3 cells. Virology 42:9–19
    [Google Scholar]
  2. Avery R. J., Levy J. A. 1978; Relationship of endogenous murine xenotropic type C virus production to spontaneous transformation of cultured cells. Journal of General Virology 39:427–435
    [Google Scholar]
  3. Avery R. J., Levy J. A. 1979; The effect of ethidium bromide on C type virus production and induction. Virology 95:277–284
    [Google Scholar]
  4. Greenberger J. S., Aaronson S. A. 1974; Morphologic revertants of murine sarcoma virus transformed non-producer Balb/3T3: selective techniques for isolation and biological properties in vitro and in vivo. Virology 57:339–346
    [Google Scholar]
  5. Hartley J. W., Rowe W. P. 1966; Production of altered cell foci in tissue culture by defective Moloney sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 55:780–787
    [Google Scholar]
  6. Macpherson I., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291–294
    [Google Scholar]
  7. Puck T. T., Kao F. T. 1967; Genetics of somatic mammalian cells. V. Treatment with BudR and visible light for isolation of nutritionally deficient mutants. Proceedings of the National Academy of Sciences of the United States of America 58:1227–1234
    [Google Scholar]
  8. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  9. Stephenson J. R., Aaronson S. A. 1972; Antigenic properties of murine sarcoma virus transformed Balb/ 3T3 non-producer cells. Journal of Experimental Medicine 139:503–575
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-49-1-105
Loading
/content/journal/jgv/10.1099/0022-1317-49-1-105
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error