1887

Abstract

SUMMARY

A hybrid between a murine myeloma cell line and spleen cells from a mouse immunized with measles has been produced. Two stable clones produce antibody with identical immunochemical and biological properties. This antibody reacts with the 76000 mol. wt. protein present in the lysates and on the surface of cells persistently infected with measles. It exhibits HAI and neutralizing activity.

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/content/journal/jgv/10.1099/0022-1317-48-2-425
1980-06-01
2022-07-01
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References

  1. Armstrong M. A., Fraser K. B., Shirodaria P. V. 1979; The sensitivity of measles virus haemolysin to acetone and the preparation of monospecific human anti-haemolysin by absorption. Journal of General Virology 44:541–544
    [Google Scholar]
  2. Bellini W. J., Trudgett A., McFarlin D. E. 1979; Purification of measles virus with preservation of infectivity and antigenicity. Journal of General Virology 43:633–639
    [Google Scholar]
  3. Bonner W. J., Laskey R. A. 1974; Film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Glorioso J. C., Smith J. W. 1977; Immune interactions with cells infected with herpes simplex virus: antibodies to radioiodinated surface antigens. Journal of Immunology 118:114–121
    [Google Scholar]
  5. Hayes E. C., Wright L. L., Zweerink H. J. 1978; Staphylococcal protein A - Sepharose columns and the characterization of measles virus-specific polypeptides in persistently infected cells. Analytical Biochemistry 91:276–282
    [Google Scholar]
  6. Howes E. L., Clark E. A., Smith E., Mitcheson N. A. 1979; Mouse hybrid cell lines produce antibodies to herpes simplex virus type I. Journal of General Virology 44:81–87
    [Google Scholar]
  7. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  8. Koprowski H., Gerhard W., Croce C. M. 1977; Production of antibodies against influenza virus by somatic cell hybrids between mouse melanoma and primed spleen cells. Proceedings of the National Academy of Sciences of the United States of America 74:2985–2988
    [Google Scholar]
  9. Kronvall G., Grey H. M., Williams R. C. 1970; Protein A reactivity with mouse immunoglobulins: structural relationship between some mouse and human immunoglobulins. Journal of Immunology 105:1116–1123
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  11. Lamb R. A., Etkind P. R., Choppin P. W. 1978; Evidence for a ninth influenza viral polypeptide. Virology 91:60–78
    [Google Scholar]
  12. McFarland H. F., McFarlin D. E. 1979; Cellular immune response to measles, mumps, and vaccinia viruses in multiple sclerosis. Annals of Neurology 6:101–106
    [Google Scholar]
  13. Tyrrell D. L. J., Norrby E. 1978; Structural polypeptides of measles virus. Journal of General Virology 39:219–229
    [Google Scholar]
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