1887

Abstract

SUMMARY

A comparative study on the infectivity and biological activities of parainfluenza virus type 2 revealed that one strain (Toshiba) showed c.p.e. with cell fusior and produced plaques in Vero cells, an established line of African green monkey kidney cells. Another strain (62-M786) of this virus, however, showed minimum c.p.e. and produced almost no plaques in Vero cells, although c.p.e. appeared and plaques were observed following addition of trypsin. The infectivity titre of the latter strain, estimated by 50% tissue culture infectious doses (TCID), was increased 10 times and plaque counts more than 6400 times by addition of an adequate concentration of trypsin; however, trypsin did not affect the infectivity of the former strain. Trypsin also increased the haemolytic activity of strain 62-M786 but not that of the Toshiba strain. These results show that the two isolates of parainfluenza virus type 2 were affected very differently by trypsin as regards infectivity, cell fusion and haemolytic activity.

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/content/journal/jgv/10.1099/0022-1317-48-2-407
1980-06-01
2021-10-23
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References

  1. Appleyard G., Maber H. B. 1974; Plaque formation by influenza viruses in the presence of trypsin. Journal of General Virology 25:351–357
    [Google Scholar]
  2. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural difference of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  3. Lazarowitz S. G., Choppin P. W. 1975; Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptide. Virology 68:440–454
    [Google Scholar]
  4. Nagai Y., Klenk H. D., Rott R. 1976; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508
    [Google Scholar]
  5. Peluso R. W., Lamb R. A., Choppin P. W. 1977; Polypeptide synthesis in simian virus 5-infected cells. Journal of Virology 23:177–187
    [Google Scholar]
  6. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
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